Identification of bacterial factors that determine the host specificity of bordetellosis.

本项目的目的是通过IVET-IP分析感染过程中细菌基因的表达，确定决定波氏杆菌病宿主特异性的细菌因素。我建立了博德特拉感染的大鼠模型，发现百日咳没有在大鼠中定植，这表明这个项目的原计划将会失败。因此，尝试了另一种方案，即通过引入具有广泛宿主范围的支气管结核分枝杆菌基因，产生定植在大鼠体内的百日咳衍生物。我期望通过这一策略可以鉴定出赋予分枝杆菌广泛宿主范围和决定宿主特异性的基因。

The aim of this project was to identify bacterial factors that determine the host specificity of bordetellosis through analyses of the bacterial gene expression during infection by IVET-IP. I established a rat model of bordetella infection, and found that B. pertussis did not colonize rats, suggesting that the original plan of this project would be unsuccessful. Therefore, tried was an alternative plan, in which B. pertussis derivatives that colonize rats are generated by introduction of genes of B. bronchiseptica that shows a wide host range. I expect that genes conferring the wide host range of B. bronchiseptica and determining the host specificity could be identified by this strategy.