Identification of bacterial factors that determine the host specificity of bordetellosis.

本项目的目的是通过对IVET-IP感染过程中细菌基因表达的分析，确定决定波氏杆菌病宿主特异性的细菌因素。我建立了一个波氏杆菌感染的老鼠模型，发现百日咳杆菌并没有在老鼠身上定植，这表明这个项目的最初计划将不会成功。因此，尝试了另一种方案，在该方案中，通过导入显示出广泛宿主范围的支气管败血杆菌的基因，产生了定植于老鼠的百日咳杆菌衍生物。我希望通过这一策略可以识别与支气管败血杆菌广泛宿主范围相关的基因，并确定宿主特异性。

The aim of this project was to identify bacterial factors that determine the host specificity of bordetellosis through analyses of the bacterial gene expression during infection by IVET-IP. I established a rat model of bordetella infection, and found that B. pertussis did not colonize rats, suggesting that the original plan of this project would be unsuccessful. Therefore, tried was an alternative plan, in which B. pertussis derivatives that colonize rats are generated by introduction of genes of B. bronchiseptica that shows a wide host range. I expect that genes conferring the wide host range of B. bronchiseptica and determining the host specificity could be identified by this strategy.