Electrophysiological analysis for pain response by the lateral bed nucleus stria terminalis

在福尔马林实验中，我们发现终纹外侧床核(BST)参与了痛反应。相反，外侧BST的功能影响福尔马林的疼痛反应。这个概念表明情感和痛苦是相互关联的。CRH神经元表达在大脑区域，如与压力相关的脑室周围下丘脑和与情绪反应流出相关的中央杏仁核。因此，外侧BST的CRH神经元可能参与了福尔马林实验中的情绪侧。为此，我们必须将CRH神经元标记为荧光，因为我们将使用膜片钳技术进行分析。因此，我们现在正在培育CRH神经元具有明亮荧光并可用于电学分析的转基因小鼠。

We discovered that the lateral bed nucleus stria terminalis (BST) is involved in the pain response during formalin test. Conversely, the function of the lateral BST influences the pain response by the formalin. This concept indicates the emotion and pain is related to each other. CRH neurons are expressed the brain area such as the periventricular hypothalamus associated with stress and the central amygdala related to outflow of the emotional response. Thus, CRH neurons in the lateral BST may be involved in emotion side during the formalin test. To this end we must label CRH neurons as fluorescence since we will analyze by patch-clamp technique. So we are now generating transgenic mouse which CRH neurons have bright fluorescence and useful for electrical analyse.