Reconstitution of the mouse spermatogonial progenitor cells from peripheral blood stem cells.

一些转基因小鼠表现出雄性不育，因此这些品系限制了大规模分析。最近的研究报道，包括单克隆小鼠抗人HLA II类抗原的良好建立的培养条件下，从外周血细胞获得的单核细胞转化为未分化的干细胞。本研究旨在探讨小鼠外周血细胞向精原祖细胞分化的潜能。本实验研究了用鼠抗人HLA-II单克隆抗体和多种因素共同作用的α-MEM培养单个核细胞，建立未分化细胞的最佳培养条件。将培养的细胞转移到免疫缺陷小鼠中以确认未分化的干细胞。体内移植后，在基底膜和基质细胞中观察到GFP阳性细胞。形成含有中胚层和内胚层的畸胎瘤。

Some transgenic mice show male infertility, thus such strains have been limited for large-scale analyses. Recent studies have reported that the inclusion of monoclonal mouse anti-human HLA class II antigen to well-established culture conditions, mononuclear cells obtained from peripheral blood cells are converted into undifferentiated stem cells. The objective of this study is to investigate the differentiation potential of mouse peripheral blood cells into spermatogonial progenitor cells. We study the optimal culture condition to establish undifferentiated cells that mononuclear cell are cultured in alfa-MEM with monoclonal mouse anti-human HLA class II antigen and some factors. The cultured cells are transferred into immunodeficient mice to confirm the undifferentiated stem cells. After transplantation in vivo, the GFP-positive cells were observed at the basement lamina and stromal cells. Also the teratoma was formed which was contained mesoderm and endoderm.