Newly vaccine strategy with mAbs against heat shock protein 90 (HSP90) on surface of antigen presenting cell (APC)

虽然HSP 90是一种胞质分子伴侣，但我们最初制备的mAb 6 H8可以检测APC上的细胞表面HSP 90。到目前为止，我们报道，与游离Ag相比，注射与Ag化学缀合的6 H8（6 H8-Ag）有效地诱导过继转移的Ag特异性CD 8 +T细胞的增殖。重要的是，这些增殖通过共同施用游离6 H8而显著增强。因此，我计划用实体瘤和人Ag模型来证实这种现象。但我们发现6 H8的亲和力受Fc部分的影响，因为它是IgG 2a抗体。然后，我们制备了另一种细胞表面HSP 90可检测的IgG 1 mAb和修饰的IgG 1型6 H8。此外，我们开始免疫针对STIP 1和HSC 70的Ab，HSC 70是我们假设的细胞表面HSP复合物的组分。虽然，到目前为止，我们还不能充分执行我们最初的项目，但已经有了用于人类Ag实验的小鼠模型，因此在完成这些Ab的制备后，我们可以重新启动我们的项目。

Although HSP90 is a cytosolic molecular chaperone, our originally produced mAb 6H8 could detect cell surface HSP90 on APCs. As so far we reported, injection of 6H8 chemically conjugated with Ag (6H8-Ag) induced proliferation of adoptively transferred Ag specific CD8+T cells efficiently, compared with free Ag. Importantly those proliferation was dramatically enhanced by co-administration of free 6H8.So I planned a project to confirm this phenomenon with solid tumor and human Ag model. But we found that 6H8's affinity was affected by Fc portion, due to it was an IgG2a Ab. Then, we prepared another cell surface HSP90 detectable IgG1 mAb and modified IgG1 type 6H8. Besides, we start immunization for Abs against STIP1 and HSC70, component of cell surface HSP complex in our hypothesis. Although, we couldn't carry out our original project enough so far, already mouse model for experiments with human Ag is available, so after finish the preparation of those Abs, we can restart our project.