The development of novel hepatic artery injection therapy for the hepatocellular carcinoma using metal nanoparticle with irradiation of external energy in animal model

由于肝细胞癌通常是在病毒感染或酒精性肝炎等肝脏障碍的基础上发展起来的，因此有必要考虑转移、复发和再治疗。因此，其治疗应具有治愈率高、创伤小的特点。本研究的目的是开发一种新的治疗肝癌的方法，使用金属纳米颗粒，如磁铁矿或二氧化钛与辐射（磁场或超声波）。 采用肿瘤移植模型和DEN致癌模型。在DEN致癌模型中肿瘤的动脉优势通过动态CT证实。建立了大鼠肝动脉注射的手术方法。证实了磁性纳米颗粒通过磁场产生热量，以及二氧化钛通过超声波产生自由基。在皮下肿瘤模型中观察到二氧化钛对肿瘤生长的抑制。

Because the hepatocellular carcinoma usually is developed based on the obstacle livers such as virus infection or the alcohol hepatitis, it is necessary to consider a metastasis, a recurrence and the re-treatment. Therefor, its treatment should have high curability and be less invasive. The purpose of this study is to develop the new therapy for the hepatocellular carcinoma using metal nanoparticles such as magnetite or titanium dioxide with irradiation (magnetic field or supersonic wave). The cancer transplant model and the DEN carcinogenesis model were used. The artery predominance of the tumor in the DEN carcinogenesis model was confirmed by dynamic CT. The surgical procedure of hepatic artery injection in rat was established. The generation of heat by magnetic field with magnetic nanoparticles and free radical by a supersonic wave with titanium dioxide were confirmed. The suppression of tumor growth was observed in the subcutaneous tumor model with titanium dioxide.