Role of the Arabidopsis TRAPPII tethering complex in protein sorting at the Trans-Golgi-Network

反式高尔基体网络（trans-Golgi network，TGN）是细胞表面信息流动和分类的主要枢纽。然而，对TGN蛋白质分选的分子机制知之甚少。该建议的重点是在胞质分裂和光反应的背景下TGN结构和功能的调节。拟南芥TRAPPII拴系复合物，被认为是作为胍交换因子（GEF）调节Rab GTdR活性的功能，正在成为胞质分裂和高尔基体后交通的中心球员。我们已经通过蛋白质组学方法确定了TRAPPII复合物的相互作用伙伴。我们建议测试的作用，这些相互作用的一个子集在蛋白质分选在细胞板。我们还将解决重新布线的TRAPPII蛋白质组响应环境刺激。我们的实验计划整合了分子遗传学，生理学，生物化学，蛋白质组学，计算和细胞生物学方法，包括超分辨率共聚焦实时成像显微镜和聚焦离子束/扫描电子显微镜，以解决TGN的结构和功能。所提出的实验的结果将大大加深我们对TGN在驱动和微调植物生长和适应中的作用的理解。

The trans-Golgi network (TGN) is a major hub for the flow and sorting of information from and to the cell surface. The molecular mechanisms underlying protein sorting at the TGN, however, are poorly understood. The focus of this proposal is on the regulation of TGN structure and function in the context of cytokinesis and of light responses. The Arabidopsis TRAPPII tethering complex, thought to function as guanidine exchange factor (GEF) regulating Rab GTPase activity, is emerging as a central player in cytokinesis and in post-Golgi traffic. We have identified interaction partners of the TRAPPII complex via proteomic approaches. We propose to test the role of a subset of these interactors in protein sorting at the cell plate. We will also address rewiring of the TRAPPII proteome in response to environmental stimuli. Our experimental plan integrates molecular genetics, physiology, biochemistry, proteomics, computational and cell biological methods, including super-resolution confocal live imaging microscopy and focused ion beam/scanning electron microscopy, to address TGN structure and function. The outcome of the proposed experiments will considerably further our understanding of the role of the TGN in driving and fine tuning plant growth and adaptation.