Structural mechanism of the activation of RIG-I by viral RNA (A13)

ATPase RIG-I检测到细胞质中存在病毒RNA，并启动一系列信号转导，从而刺激先天免疫反应。我们首次获得了RIG-I识别病毒RNA的结构洞察力。在这项提议中，我们想要推导出一个完整的结构机制，由病毒RNA激活RIG-I。在合作中，我们通过结构、生化和体内功能的方法解决了RIG-I如何与病毒RNA结合以及RNA和ATP结合如何在RIG-I中诱导信号构象从而刺激先天性免疫反应。

The ATPase RIG-I senses the presence of viral RNA in the cytoplasm and initiates a signal transduction cascade that leads to stimulation of the innate immune response. We have obtained first structural insights into recognition of viral RNA by RIG-I. In this proposal we want to derive a complete structural mechanism of activation of RIG-I by viral RNA. In collaboration we address by structural, biochemical and in vivo functional approaches, how RIG-I binds viral RNA and how RNA and ATP binding induce a signaling conformation in RIG-I that leads to stimulation of the innate immune response.