Field-assisted assembly of the DNA networks for electronics and biologydcal applications

在JSPS奖学金的最后一年（2018-2019），我们建立了新的工具和方法来解决研究过程中遇到的技术困难。最重要的是，我们成功地优化了缓冲条件，使连接的λ-DNA（~100微米长）在aptes涂覆的玻璃载玻片上使用带反电极的纳米吸管沉积。在荧光显微镜下对图案线进行成像。我们成功地实施了新的策略，克服了水和液态金属在通道横截面直径从~500 um减小到~100 nm时的显著阻力。构建了一个新的移液器支架，可以同时应用高压（高达~200 Atm），加热（高达~500℃）和高压（高达10 kV）到移液器。使用新的支架，我们能够通过纳米吸管通道挤出GaIn合金。建立了用化学镀法在玻璃毛细血管内部镀金的方案，并取得了成功的可重复性结果。金涂层纳米吸管将用于各种应用。

In the final year of the JSPS fellowship (2018-2019), we established new tools and methodologies to address technical difficulties encountered during our research. Most significantly, we succeeded in optimizing buffer conditions for the deposition of ligated λ-DNA (~100 µm in length) on APTES-coated glass slides using the nanopipette with a counter electrode. Imaging of the patterned lines was done in a fluorescence microscope.We successfully implemented new strategies to overcome significant resistant to flow of water and liquid metals as channel cross section decreases in diameter, from ~500 um to ~100 nm:1. A new pipette holder was constructed, enabling simultaneous application of high pressure (up to ~200 Atm), heating (up to ~500 C) and high voltage (up to 10 kV) to pipette. Using the new holder, we were able to extrude GaIn alloy through the nanopipette channel.2. Protocol for coating of the inside of glass capillaries with gold using electroless plating was established, with successful reproducible results. Gold coated nanopipettes will be used for various applications.