自然環境下でのマングローブのゲノム・エピゲノム解析

红树林群落对恶劣的海岸环境具有高度的适应性，这使它们成为研究逆境耐受性和适应恶劣环境的分子机制的宝贵资源和模型。通过对全基因组基因表达和甲基化谱的综合分析，我们研究了表观遗传变异是否有助于这些抗性树木应对恶劣的栖息地。我们首先利用长读测序技术（PACBIO）和更传统的平台（Illumina Hi-seq和matepair）相结合，为具有代表性的红树林物种Bruguiera gymnorhiza建立了高质量的参考基因组。在此基础上，研究了不同生境、不同应激水平下的甲基化变异在个体间的分布，以及甲基化变异与个体形态差异和基因表达的关系。我们还对相同的群体进行了RNA-seq分析和基因表达分析，并研究了这些样本中转录组和甲基化的关系。本项目建立的高质量参考基因组序列为这些重要植物的epi基因组动力学提供了新的见解，并为未来的研究提供了基础，不仅在红树林，而且在其他树种。

Mangrove communities show high adaptability to harsh coastal condition that makes them a valuable resource and model for studying the molecular mechanisms behind stress tolerance and adaptation to harsh environments. Using a comprehensive analysis of genome-wide gene expression and methylation profiles we investigated if epigenetic variations contribute to help these resistant trees cope with their harsh habitats. We first produced a high-quality reference genome for a representative mangrove species Bruguiera gymnorhiza using the combination of long read sequencing technology (PACBIO) supplemented by the more conventional platforms (Illumina Hi-seq and matepair). We then investigated how methylation variation is distributed among individuals from different habitats in different level of stress, and how this variation is correlated with their morphological differences and gene expression. We also performed RNA-seq analysis and gene expression analyses for the same populations and investigated the association of transcriptome and methylation among these samples. High-quality reference genome sequences built in this project offers new insights into the (epi)genome dynamics of these important plants and provide a basis for future studies, not only in mangrove but also in other tree species.