Interplay between speciation and adaptation: an 'Omics' study on the effects of human impacts on Mediterranean and Indo-Pacific Zoantharia

使用分子方法和高通量测序(HTS)技术，Poliseno博士探索了大西洋-地中海兽类Savaglia savalia的种内多样性和种群结构。波利塞诺博士研究了与印度-太平洋海绵相关的兽类Umy ayantus及其宿主的遗传多样性。最后，鉴于在野外观察到的高度丰富性和多样性，该研究员对从冲绳岛和日本南部其他岛屿收集的柳珊瑚进行了生物多样性调查。对于Savalia Savaglia的种群基因组学，Poliseno博士发现，从地中海盆地(法国、黑山、阿普利亚和撒丁岛-意大利)和东大西洋(葡萄牙)的5个不同地理区域收集的个体分为三个不同的聚类群。我们的结果表明，无性繁殖可能解释了某些种群中克隆个体的存在。波利塞诺博士没有发现地理距离和遗传结构之间的任何关联，而我们观察到，较深的种群在遗传上更相似，而来自较浅地区的种群彼此之间非常不同。对海绵相关的兽类和它们的宿主的多样性分析表明，缺乏物种特有的联系。特别是，Poliseno博士发现，就所调查的标本而言，与Unimayantus有关的海绵至少属于五个属(例如，Agelas、Clatrea、Cliona、Hymeniacidon和Spirastrella)。

Using a molecular approach and High Throughput Sequencing (HTS) technologies, Dr. Poliseno explored the intra-specific diversity and population structure of the Atlantic-Mediterranean zoantharian Savaglia savalia. Dr. Poliseno investigated the genetic diversity of the Indo-Pacific sponge associated zoantharian Umimayanthus and their hosts. Finally, given the high abundance and diversity observed in the field, the fellow conducted a biodiversity survey of the gorgonian octocorals collected from Okinawajima island and other islands of southern Japan.For population genomics of Savalia savaglia Dr. Poliseno found that individuals collected from 5 geographical distinct areas across the Mediterranean basin (France, Montenegro, Apulia and Sardinia - Italy) and eastern Atlantic (Portugal) grouped into three distinct clusters. Our results showed that asexual reproduction may possibly explain the presence of clonal individuals within some populations. Dr. Poliseno did not find any correlation between geographic distance and genetic structure, whereas we observed that populations at greater depths are genetically more similar, whereas those from shallower areas are very distinct from each-other.The analyses on the diversity of the sponge associated zoantharian Umimayanthus and their hosts suggest the lack of species-specific association. In particular Dr. Poliseno found that, for the specimens investigated, the sponges associated with Unimayanthus belong to at least five genera (e.g. Agelas, Clathria, Cliona, Hymeniacidon and Spirastrella).