Study on rhizosphere of arsenic hyperaccumulators forward to develop an efficient phytoremediation technology

研究工作按计划顺利进行。在这一年里，我继续关注砷(As)超积累植物，它展示了一种很有前途的植物修复策略，以及通过将根际细菌与它们结合而产生的额外潜力。我已成功地从凤尾草和多裂凤尾草中分离到巴西铜绿假单胞菌r507和万古威假单胞菌m318菌株作为超积累菌。这些菌株表现出一些潜在的植物修复特性，如较高的耐受性，具有aioA基因，具有氧化、铁载体和IAA分泌，对油松有较高的亲和力。对菌株M318进行了长期的田间试验，验证了菌株M318对AS植物修复的稳定性和可行性，这不仅是为了恢复地上植被，也是为了确保地下土壤及其伴生微生物处于未受污染的状态和良好的生物活性。此外，探索植物与伴生微生物之间相互作用的紧密系统表明，油松是改变根际细菌群落结构的主要驱动力。我发表了3篇论文，并参加了几次国际会议和国家会议。学术交流为我的研究提供了启发性的思想和想法。

The research went smoothly as planned. In this year, I continually focused on arsenic (As) hyperaccumulators which showed a promising strategy as As phytoremediation and additional potential by combining rhizobacteria with them. I have successfully isolated Cupriavidus basilensis strain r507 and Pseudomonas vancouverensis strain m318 strains from Pteris vittata and Pteris multifida (As hyperaccumulators). These strains showed several promising potential traits that may facilitate As phytoremediation, such as higher As tolerance, with aioA-genes, As oxidation, siderophore and IAA secretion, high affinity to P. vittata. I tried to do the long-term field trial to test the stability and feasibility of strain m318 for improvement of As phytoremediation, which suggested is not only to restore the aboveground vegetation but also to make sure underground soil and associated microorganisms with a state of uncontaminated and good bioactivity. Furthermore, the close system to explore the interaction between the plants and associated microorganisms demonstrated the P. vittata was the major drive force to shift the rhizobacterial community structure. I published 3 papers and attended several international conferences and national conferences. Academic communication provides the enlighten thoughts and ideas about my research.