Study on metabolism/physiological functions of retinoic acid in metamorphic larvae of the Pleuronectiformes

1. RT-PCR证实bco 1 l在肠道中被RA处理下调。cyp 26和dhrs 3在两侧皮肤、脊椎和鳃中表达上调。数据表明，RA暴露引起的畸形与该机制系统相关。成功将质粒转移到比目鱼组织和竹鳎卵巢卵中，为将CRISPR/Cas9系统应用于比目鱼的发育生物学不对称性研究奠定了基础.编码维甲酸受体的基因在G期比目鱼中普遍表达。两侧皮肤和椎骨中rars的表达提示皮肤和椎骨对RA诱导有反应。G期RA诱导的骨和皮肤畸形与这些rar的表达有关。

1. RT-PCR confirmed bco1l was down-regulated by RA treatment in the intestine. Members of cyp26 and dhrs3 were up-regulated in both sides of skin, vertebra and gills. The data suggest malformations caused by RA exposure is systemically related to this mechanism.2. The success of transferring plasmid to flounder tissues and bamboo sole ovary eggs provides the basis for technique using CRISPR/Cas9 system in flatfish, which distribute to developmental biology in asymmetry study.3. Genes encoding retinoic acid receptors expressed ubiquitously in G-stage flounders. The expression of rars in both sides of skin and vertebra suggest the skins and vertebra are responsive to RA induction. Bone and skin malformation caused by RA induction at G-stage is related to the expression of these rars.