BAT thermogenesis-triggered organ cross-talk through the exosomal microRNA

为了了解白细胞介素-6 （IL-6）和肌生长抑制素（GDF - 8）对肌细胞中microRNA 122 （miR-122）表达的影响，C2C12成肌细胞分化14至16天，并显示出肌管形成，用两种不同浓度的上述细胞因子分别处理6小时。处理后，收集细胞的microRNA并测量。25ng IL-6显著增加C2C12 miR-122的表达（%），而更高的100ng处理浓度与对照组相比仅增加C2C12 %。为了确定IL-6对C2C12细胞中miR-122表达的影响，我检查了时间和剂量反应。5ng、10ng和25ng IL-6剂量对C2C12的影响无显著性差异。相比之下，观察到对IL-6治疗的时间反应有显著差异。2小时后，miR-122的表达比治疗开始时高56%。在治疗后4小时，观察到miR-122表达增加53%，而在8小时后，C2C12细胞中miR-122表达下降31%。这表明心肌细胞miR-122表达对IL-6有显著的时间响应。另一方面，GDF8处理没有对C2C12 miR-122的表达产生任何显著影响

To understand the effect of batokines: interleukin-6 (IL-6)and myostatin (GDF 8), on microRNA 122 (miR-122) expression in myocytes, C2C12 myoblasts differentiated for 14 to 16 days and showing myotube formation were treated with two different concentrations of each of the mentioned batokines for 6 hours. After treatment, the microRNA of the cells were harvested and measured. 25ng of IL-6 significantly increased C2C12 miR-122 expression (%) unlike the higher 100ng treatment concentration which only increased the C2C12 by % compared to the control group.To determine the potency of IL-6 effect on miR-122 expression in C2C12 cells, I checked the time- and dose-responses. The effects of 5ng, 10ng and 25ng IL-6 doses on C2C12 were not significantly different from each other. In contrast, a significant difference in the time-response to IL-6 treatment was observed. After 2 hours, miR-122 expression was 56% higher than at the start of the treatment. At 4 hours after treatment, a 53% increase in the miR-122 expression was observed whereas after 8 hours, there was a 31% drop in the miR-122 expression in the C2C12 cells. This indicates a significant time-response of myocyte miR-122 expression to IL-6.On the other hand, GDF8 treatment did not produce any significant effects on C2C12 miR-122 expression