Molecular characterization of duckweeds at the level of clones, species, genera and subfamily by amplified fragment length polymorphism and plastidic DNA sequencing

浮萍(天南星科-浮萍亚科)是一小群植物(37种)，在生物监测(自2006年以来存在一项ISO协议)和植物修复方面越来越重要。由于这些植物的大多数特征都大大减少，在形态水平上鉴定物种甚至对专家来说都是一个挑战。因此，应大力开发和应用分子标记。来自浮萍属所有属和种的约150个已确认身份的克隆将通过扩增片段长度多态性(AFLP)和三个可塑性DNA区域(TML-trnF、TMS-trnG和RP/16)的测序来实现以下目标：(1)开发一种有效的方法来确定与物种有关的新分离的植物样本的身份或确认它们的身份。(2)浮萍克隆的分子特征(条形码)，用于生物监测、植物修复和遗传转化或确认其克隆特性。(3)应用所获得的数据来检验和改进天南星科-浮萍亚科系统发育关系的现有知识。

Duckweeds (Araceae-Lemnoideae) are a small group of plants (37 species) with increasing importance in biomonitoring (since 2006 an ISO protocol exists) and phytoremediation. Because of the great reduction of most characteristics of these plants, identification of species on a morphological level is a challenge even for specialists. Therefore, molecular markers should be developed and applied. Approximately 150 clones with confirmed identity from all genera and species of the duckweed group will be characterised by amplified fragment length polymorphism (AFLP) and by sequencing three plastidic DNA regions (tmL - trnF, tmS - trnG, and rp/16) to reach the following goals: (1.) Development of an effective method to determine the identity of newly isolated plant samples concerning the species or to confirm their identity. (2.) Molecular characterisation (bar coding) of duckweed clones for application in biomonitoring, phytoremediation and genetic transformation or to confirm their clonal identity. (3.) Application of the obtained data in order to test and improve the present knowledge of phylogenetic relationships in Araceae-Lemnoideae.