study of Proteoglycans in Brains of Elderly Humans

HSPG抗体的免疫活性在含有中央淀粉样核的NPs的经典斑块和没有淀粉样核的原始斑块中都清楚地显示出来。“无定形”或“弥漫”斑块也呈HSPG免疫阳性。此外，即使在不含淀粉样蛋白的脑膜和脑内小血管/毛细血管中，也几乎总是观察到HSPG免疫阳性。其他4种单抗(DSPG：6-B-6，CSPG：CS-56，Di-4S：2-B-6，Di-6S：3-B-3)仅限于两种类型的NPS(经典型和原始型)。DSPG和CSPG在正常老年人和ADA脑的胶质细胞和细胞外基质中均有免疫活性。用免疫组织化学方法检测了正常老年脑和阿尔茨海默病患者脑细胞内和细胞外(幽灵)NFT和非缠结神经元中HSPG、DSPG和CSPG的表达。与其他四种抗体相比，HSPG免疫反应在所有三种类型的NPs(弥漫型、原始和经典型NPs)中均呈强阳性。然而，阿尔茨海默病患者脑内的免疫反应强度与正常老年脑相比并无明显差异。

Immunoactivities for antibody to HSPG were clearly demonstrated in classical plaques of NPs containing a central amyloid core and also in primitive plaques without an amyloid core. The 'amorphous' or 'diffuse' plaques were also strongly HSPG-immunopositive. In addition, HSPG-immunopositivity was almost always observed even in meningeal and intracerebral small vessels/capillaries which did not contains amyloid. Immunostaining of four other monoclonal antibodies (DSPG : 6-B-6, CSPG : CS-56, Di-4S : 2-B-6, Di-6S : 3-B-3) was limited to two types of Nps (classical and primitive types). Immunoactivity of DSPG and CSPG was easily identified in glial cells and extracellular matrix of normal aged and ADA brains. HSPG,DSPG and CSPG were immunohistochemically detected in both intracellular and extracellular (ghost) NFTs and nontangle-bearing neurons in normal aged brains and the AD brain. Immunoreaction with HSPG was strongly immunopositive in all three types of NPs (diffuse, primitive and classical types of NPs) in contrast to the four other antibodies. However, no distinct difference in immunoreactive intensity was sen in the AD brain compared to normal aged brains.