The establishment of total risk assessment method for water pollution included sediment.

近年来，重金属、农药、除草剂、洗涤剂等对水环境的污染日益严重。此外，在总体风险评估中，还需要对沉积物中积累的化学品的毒性进行主要规定。为了评估沉积物中化学物质的污染，我们建立了一个新的生物测定系统，使用秀丽隐杆线虫（C.elegans）和细胞培养。因此，利用RT-PCR技术对水稻基因组进行分析，将有助于筛选环境污染及其胁迫条件。在这项研究中，我们试图评估金属硫蛋白、细胞色素P450 IA和血红素加氧酶的mRNA水平与水环境污染的关系。在细胞培养研究中，我们发展了几种底泥毒性的生物测定方法，如青蒿蓝<TM>法、DNA放射法和彗星试验。我们建立的这些生物标志物是环境化学品毒性的很好的指示剂。另一方面，卵黄蛋白原mRNA的诱导能力也可能提示其化学物质具有内分泌干扰作用。这是通过免疫、代谢、形态发生、神经和生殖等功能的调节来实现的，本研究结果表明，所建立的方法是一种有效的手段，可作为沉积物环境污染评价的报警系统，在实验室和野外工作中使用。

Recently, it becomes very serious problem that an aquatic environmental pollution caused by heavy metals, pesticides, herbicide and detergents. In addition, a major provision of the toxicity for there chemicals which accumulate within sediment also required for the total risk assessment. For assessing the sediment contamination with chemicals, we established a new bioassay system using Caenorhabditis elegans (C.elegans) and a cell culture. It is considered that the analysis of mRNAs using RT-PCR will enable us screen the environmental pollution and its stress conditions. In this study, we tried to evaluate mRNA levels of metallothionein, cytochrome P450IA and heme oxygenase for aquatic environmental pollution. In cell culture study, we developed some bioassaies for toxicity of sediment such as aramar Blue^<TM> assay and DNA radder and comet assay. These biomarkers which we established are very good indicator for the toxicity for environmental chemicals. On the other hand, the induction ability of vitellogenin mRNA may also suggest that its chemical will be anxious for the action of endocrine disruption. This is accomplished through regulation of irmmune, metabolic, morphogenic, neural and reproductive functions.The results of my study, in conclusion, the established method is a useful means as an alarm system both in the laboratory and field work for assessment of environmental pollution for sediment.