Precise Distinctions between Very Immature T-linegae and NK-lineage Neoplasms

本课组在未成熟t细胞肿瘤T-ALL/LBL的表型和基因分析方面做了重要工作（Blood 82: 207,1993; Am J Hematol 45: 150,1994; ibid 45: 310,199 4; ibid 49: 6.1995）。人们强烈怀疑，前胸腺期的CD7+CD2+亚群，被认为是非常不成熟的t谱系的一个亚群，似乎来自nk谱系的一个非常不成熟的阶段（Blood 89: 4665,1997）。对CD7+CD2+亚群的详细研究是本研究的主要目标。然而，与CD7+CD5+组相比，该亚组的发病率非常低，因此在研究期间未遇到任何此类新病例。（在撰写本报告的过程中，已经经历了一个案例，目前正在进行调查。）我们在10株表达TCRalpha_beta的T-ALL/LBL细胞株、3株表达TCRgamma_delta的T-ALL/LBL细胞株和1株成熟NK细胞株YT.[1]中研究了pre - talpha（前t细胞受体α）的表达分布tcrα链表达后，表达足量的preTalpha在TCR β基因单克隆重排的3个表达TCRgamma_delta的T-ALL/LBL细胞系中，preTalpha意外表达。这两个是相当新的观察结果。

Important works (Blood 82 : 207,1993 ; Am J Hematol 45 : 150,1994 ; ibid 45 : 310,199 4 ; ibid 49 : 6.1995) have been done by our group regarding phenptypic and gene ana Lysis of T-cell neoplasms derived from immature stages, T-ALL/LBL.It was strongly suspected that CD7+CD2+ subset of pro-thymic stage, which had been regarded as a subset of very immature T-lineage, seemed to be derived from an very immature stage of NK-lineage (Blood 89 : 4665,1997). The detailed research of this CD7+CD2+ subgroup is the major goal of this research. However, the incidence of this subgroup has been so low compared with CD7+CD5+ group that any such fresh case has not been encountered during the research peripod. (During writing this report, one case has been experiences, and the investigation is now going on.)The distribution of the expression of preTalpha (pre T-cell receptor alpha) has been studied in 10 TCRalpha_beta expressing T-ALL/LBL cell lines, 3 TCRgamma_delta expressing T-ALL/LBL cell lines and a mature NK cell line, YT.[1] After expressing TCRalpha chain, enough amount of preTalpha is expressed.[2] In the 3 TCRgamma_delta expressing T-ALL/LBL cell lines, which have the monoclonal rearrangement of TCR beta gene, preTalpha are unexpectedly expressed.These two are quite new observations.