Function and mechanism of the lysosomal polypeptide transporter ABCB9 (P09)

ABCB9形成同源二聚体复合体，功能上分为核心复合体和N端跨膜结构域TMD0，负责溶酶体的定位和与LAMP-1/2的相互作用。我们将通过核磁共振光谱确定其分子结构。此外，还将调查TMD0与其交互伙伴之间的接口。用细胞生物学方法研究ABCB9溶酶体转运的机制。将用单分子技术和快速动力学分析多肽的结合和释放以及三磷酸腺苷的水解。

ABCB9 forms a homodimeric complex, which can functionally be divided into the core complex and the N-terminal, transmembrane domainTMD0, responsible for lysosomal localization and interaction with LAMP-1/2. We will determine the molecular structure by NMR spectroscopy. Further, the interface between TMD0 and its interaction partners will be investigated. With cell biological methods the machinery for lysosomal trafficking of ABCB9 will be studied. Peptide binding and release as well as ATP hydrolysis will be analyzed by single molecule techniques and fast kinetics.