Immunohistochemical study of click sensitivity of the vestibular system

前庭系统敲击敏感性的免疫组织化学研究[简介]神经生理学研究表明，哺乳动物的前庭神经元可以对响亮的敲击做出反应。这些研究支持人类短声诱发的肌源性电位可能起源于前庭。虽然单个单位的研究提供了一个强有力的证据表明前庭神经元对短声的敏感性，但要了解前庭系统中短声敏感神经元的分布并不容易。免疫组织化学研究将提供补充的结果，在此，我们报告关于o-Fos蛋白诱导的前庭系统敏感性的免疫组织化学结果。[材料与方法]成年Wistar大鼠(180~200g)接受听性脑干反应阈值以上90dB短声刺激40min。刺激结束后，用卡莫夫斯基液(2.5%多聚甲醛和0.4%戊二醛)灌流固定。冰冻的大鼠脑组织被切成冠状切片。对标本进行c-Fos蛋白检测。第一个抗体是抗c-Fos lgG(兔)。用生物素标记的抗兔GGG(痛风)和生物素标记的过氧化物酶检测组织结合抗血清，再用二氨基联胺和过氧化氢显色过氧化物酶。[结果]耳蜗核、上橄榄核、梯形小体、下丘等听觉通路对c-Fos蛋白有较强的诱导作用。在前庭核团中，c-Fos蛋白在前庭外侧核、脊髓核和内侧核均有表达。[结论]这些结果证实了有关前庭神经元对短声的敏感性的神经生理学研究。

Immunohistochemical study of click-sensitivity of the vestibular system[Introduction] Neurophysiological studies revealed that vestibular neurons in mammals could respond to loud click. These studies have supported that click-evoked myogenic potentials in humans could be of vestibular origin. While single unit studies provide a strong evidence of sensitiveness of vestibular neurons to click, it is not easy to get knowledge about distribution of click-sensitive neurons in the vestibular system. Immunohistochemical study would provide complemental findings, Herein we report immunohistochemical findings about sensitiveness of the vestibular system by o-Fos protein induction. [Materials ＆ Methods] Adult wister rats (180 to 200 g) received click stimulation of 90 dB above the ABR threshold for 40 minutes. After the stimulation, rats were perfused and fixed by Kamovsky's solution (2.5% paraformaldehyde and 0.4% glutaraldehyde). Frozen brainstems of rats were cut into coronal sections. The specimens were reacted for c-Fos protein. First antibody was anti-c-Fos lgG (rabbit). The tissue-conjugated anti-serum was detected with biotinylated anti-rabbit lgG (gout) and avidin-biotinylated peroxidase followed by the visualization of peroxydase with diaminobenzidine and hydrogen peroxide. [Results] The auditory pathway including the cochlear nuclei, the superior olive, the trapezoid body, the inferior colliculi showed strong induction at c-Fos protein. In the vestibular nuclei, induction of c-Fos protein was observed in the lateral, spinal, and medial vestibular nuclei. [Conclusion] These results confirmed neurophysiological studies concerning sensitiveness or vestibular neurons to click.