Identification of dermal papilla derived agents which transform the terminalhair to vellus hair

（一）.从患有雄激素性脱发的男性受试者的秃头病变和非秃头枕部病变获得的切除毛囊培养两种类型的毛乳头细胞。提取毛乳头细胞总RNA，以oligo-dT tailed pUC 19为引物，构建毛乳头细胞3 '端cDNA文库。对于两个文库，每个菌落随机挑取约1000个，测定每个cDNA的核苷酸序列，并将基因表达谱与数据库（BodyMap）进行比较。我们将其分为3类：1）在正常对照DPC中高表达而在秃毛DPC中不表达的新基因。2)新的基因在秃毛DPC中高度表达，而在正常对照DPC中不表达。3)已知的基因，其表达在两者之间高度不同。I型胶原蛋白、纤连蛋白和骨连接蛋白，它们在两个文库中被克隆了10次或更多次，这意味着DPC大量产生这些细胞外基质。在sillicob比较的基因鉴定对照DPC特异性和秃头DPC特异性基因，所有这些都是特异性表达的毛乳头细胞。最后，我们选择了8个候选基因，其表达谱和功能正在通过开发这些基因的敲除动物进行研究。（二）、甲状腺激素受体的表达在毛囊检查。所有构成毛囊的细胞均呈T3受体阳性。此外，T3对体外培养毛囊细胞生长的促进作用在生理浓度时最强。（三）、获得了抗人毛乳头的单克隆抗体。其中一个被证实能识别一种新的角蛋白。

(1). Two types of dermal papilla cells were cultured from the excised hair follicles obtained from the bald lesion and non bald occipital lesion of a male subject afflicted with an androgenic alopecia. Then total RNAs were extracted from those two tupes of dermal papilla cells (DPCs), and 3'-directed cDNA libraly were constructed using oligo-dT tailed pUC19 as a primer. Approximately 1000 each colonies were picked up randomly for both libraries, and nucleotide sequence of each cDNA was determined, and gene expression profiles were compared with the data base (BodyMap) ose. The we categorize them into 3 groups ; 1) new genes highly expressed in normal control DPCs but not in the bald DPCs. 2) new genes highly expressed in the bald DPCs but not in normal control DPCs. 3) known genes whose expression is highly different between the two. Type I collagen, fibronectin, and osteonectin, which were cloned ten times or more in both libraries, which means that DPCs abundantly produce those extracellular matrix. In sillicob comparison of the genes identified control DPC-specific and bald DPC-specific genes, all of which were speciffically expressed in dermal papilla cells. Finally we chose 8 candidate genes and whose expression profiles and functions are now being studied by means of developing knock-out animals of those genes.(2). Thyroid hormone receptor expression was examined in the hair folicle. All cells which compose the hair follicle were T3 receptor positive. In addition, the activity of T3 on the growth of cultured hair follicle cells is highest at the phisiological concentration.(3). Monoclonal antibodies against human dermal papilla were obtained. One of which was confirmed to recognize a new keratin.