Development of Artificial Photosynthetic System by Functional Antbody Proteins.

Mab 13 -1抗体L链的过氧化物酶活性远高于TCPP Fe（III），且13- 1 L链与TCPP Fe（III）复合物的过氧化物酶反应最适温度为90 ℃，而单独的TCPP Fe（III）的温度为60 ℃。突变催化抗体的表征显示，（残基编号115-146）对于高热稳定性和亲水区域是重要的（残基编号147-189）用计算机软件AbM预测了该抗体的三级结构，并与多枝节霉菌过氧化物酶的结构进行了比较，确定了对酶催化活性起重要作用的His和Arg残基在L链的抗原结合位点（His 38、His 94和Arg 96）和互补决定区（CDR）中发现了3个保守的氨基酸残基，并开发了一种新的具有过氧化物酶活性的催化抗体（L-zyme）。 ...更多信息 我们尝试用单克隆抗体L链与卟啉分子的复合物来增强光诱导产氢的能力，当L链与TCPP Zn（II）的复合物用于甲基紫精的还原时，复合物作为光敏剂的能力优于TCPP Zn（II）。电子转移与氢化酶偶联，配合物比TCPP Zn（II）能产生更多的氢在此基础上，我们研究了光动力学疗法（PDT）诱导肿瘤细胞凋亡的作用。PDT能有效地诱导HeLa细胞凋亡，其信号转导机制与紫外线（UV）相似而PDT则未观察到DNA断裂和INK激酶的激活。基于这些实验数据，开发更有效的诱导癌细胞凋亡的方法是必要的。

The antibody L chain from Mab13-1 exhibits much higher peroxidase activity than TCPP Fe(III) alone.Furthermore, optimum temperature of the peroxidase reaction by the complex of 13-1L chain and TCPP Fe(III) was 90ﾟC, while that of the TCPP Fe(III) alone was 60ﾟC.Characterization of the mutant catalytic antibodies revealed that the hydrophobic region in the constant region (residue number 115-146) is important for high thermostability and the hydrophilic region (residue number 147-189) is important for high activity.Tertiary structure of this L chain catalytic antibody was predicted using a computer software AbM and the structure was compared with the strucure of the peroxidase from Arthromyces ramosus.Important His and Arg residues for the calytic activity were conserved in the antigen binding site of the L chain (His38, His94 and Arg96) and they were found in complementarity determining regions (CDR).Likewise, we have developed a new catalytic antibody (L-zyme), which exhibits peroxida … More se activity from anti-porphyrin antibody L-chain.We attempted to enhance photoinduced hydrogen production using a complex of the monoclonal antibody L chain and porphyrin molecule.When the complex of L chain and TCPP Zn(II) was used for reduction of methyl viologen, the comoplex exhibited better ability as a photosensitizer than TCPP Zn (II) alone.The electron transfer was coupled with hydrogenase and the complex could lead to production of more amount of hydrogen than TCPP Zn (II).Triplet lifetimes were measured and the complex exhibited longer lifetimes.Based on these data we studied apoptosis of cancer cells induced by photodynamic therapy (PDT) using hematoporphyrin molecule.PDT induced apoptosis very effectively when HeLa cells are treated and signal transduction mechanism of PDT was similar to that of ultraviolet (UV) but DNA fragmentation and activation of INK kinase could not be observed in the case of PDT.Development of more effective method to induce apoptosis of carcinoma cells is in progess based on these experimental data Less