Studies about protein structural alteration switching using in vivo mimic system.

使用体内模拟系统进行蛋白质结构改变转换的研究。

基本信息

项目摘要

Protein aggregation is facilitated by a partial unfolding during thermal or oxidative stress and byalterations in primary structure caused by mutation. Protein aggregation is a major problem in biomedical and biotechnological fields. In addition, with a growing expansion of genomic data, protein purification is one of the keys to perform post-genomic researches. In our living cells, protein aggregates are highly associated with fatal diseases, such as Alzheimer's, Creutzfeldt-Jakob, Huntington's and Parkinson's diseases. In this research, we focus on amine compounds as additive for preventing heat-induced aggregation of lysozyme, as a model protein. Furthermore, we investigated the fibrillogenesis of amyloid β and β_2-microglobulin in the presence of the additives.We investigated whether small amine compounds prevent heat-induced aggregation and inactivation of lysozyme and fibrilization of β_2-microglobulin. The following conclusions are proposed.(i)o-Diaminocyclohexane and p-phenylenediamine prevented heat-induced aggregation andinactivation of lysozyme. The inactivation rates of lysozyme in the presence of the additives were 3-fold slower than that in the absence of additives. (ii)p-Diaminocyclohexane and spermine prevented fibrilization of β_2-microglobulin. The rate of the fibrils in the presence of the additives is 4-fold slower than that in the absence of additives. These data provided new candidates for small additives to prevent protein aggregation.
蛋白质聚集是由热或氧化应激过程中的部分解折叠和由突变引起的一级结构的改变而促进的。蛋白质聚集是生物医学和生物技术领域的一个主要问题。此外,随着基因组数据的不断扩大,蛋白质纯化是进行后基因组研究的关键之一。在我们的活细胞中,蛋白质聚集体与致命疾病高度相关,如阿尔茨海默氏症、克雅氏病、亨廷顿病和帕金森氏病。本研究以溶菌酶为模型蛋白,研究了胺类化合物对溶菌酶热诱导聚集的抑制作用。此外,我们还研究了添加剂对淀粉样蛋白β和β_2-微球蛋白纤维化的影响,以及小分子胺类化合物对溶菌酶热诱导聚集失活和β_2-微球蛋白纤维化的抑制作用。兹提出以下结论。(1)邻-环己二胺和对苯二胺能阻止溶菌酶的热诱导聚集和失活。在添加剂存在下的溶菌酶的失活速率比在没有添加剂的情况下慢3倍。(ii)对二氨基环己烷和精胺可抑制β_2-微球蛋白的纤维化。在添加剂存在下的原纤维的速率比在不存在添加剂的情况下的原纤维的速率慢4倍。这些数据为防止蛋白质聚集的小添加剂提供了新的候选物。

项目成果

期刊论文数量(68)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Dissolution of protein aggregation by small amine compounds
小胺化合物溶解蛋白质聚集
Surface histidine residue of archaeal histone affects DNA compaction and thermostability
  • DOI:
    10.1016/s0378-1097(03)00358-6
  • 发表时间:
    2003-07-15
  • 期刊:
  • 影响因子:
    2.1
  • 作者:
    Higashibata, H;Siddiqui, MA;Fujiwara, S
  • 通讯作者:
    Fujiwara, S
Screening for stable mutants with amino-acid pairs substituted for the disulfide bond between residues 14 and 38 of bovine pancreatic trypsin inhibitor (BPTI).
筛选用氨基酸对取代牛胰蛋白酶抑制剂 (BPTI) 残基 14 和 38 之间的二硫键的稳定突变体。
  • DOI:
  • 发表时间:
    2002
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Yoshihisa Hagihara;Kentaro Shiraki;Tsutomu Nakamura;Koichi Uegaki;Masahiro Takagi;Tadayuki Imanaka;Noboru Yumoto
  • 通讯作者:
    Noboru Yumoto
Amino acid esters prevent thermal inactivation and aggregation of lysozyme
  • DOI:
    10.1021/bp049769w
  • 发表时间:
    2005-03-01
  • 期刊:
  • 影响因子:
    2.9
  • 作者:
    Shiraki, K;Kudou, M;Takagi, M
  • 通讯作者:
    Takagi, M
Mutational effects on O6-methylguanine-DNA methyltransferase from hyperthermophile contribution of ion-pair network to protein thermostability.
离子对网络的超嗜热菌对蛋白质热稳定性的贡献对 O6-甲基鸟嘌呤-DNA 甲基转移酶的突变影响。
  • DOI:
  • 发表时间:
    2004
  • 期刊:
  • 影响因子:
    0
  • 作者:
    An;C.-I.ほか;S.Nishikori et al.
  • 通讯作者:
    S.Nishikori et al.
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TAKAGI Masahiro其他文献

Observing system simulation experiment for radio occultation measurements of the Venus atmosphere among small satellites
小卫星金星大气射电掩星测量观测系统模拟实验
Limb fitting and cloud tracking for the study of the Venus atmosphere
用于金星大气研究的肢体拟合和云跟踪
  • DOI:
  • 发表时间:
    2013
  • 期刊:
  • 影响因子:
    0
  • 作者:
    OGOHARA Kazunori;KASHIMURA Hiroki;KOUYAMA Toru;SATO Naoki;TAKAGI Masahiro;IMAMURA Takeshi;HORINOUCHI Takeshi
  • 通讯作者:
    HORINOUCHI Takeshi
Mathematical analysis for the system in electro-magneto-hydrodynamics
电磁流体动力学系统的数学分析
  • DOI:
  • 发表时间:
    2022
  • 期刊:
  • 影响因子:
    0
  • 作者:
    SUGIMOTO Norihiko;ABE Mirai;KIKUCHI Yukako;HOSONO Asako;ANDO Hiroki;TAKAGI Masahiro;GARATE LOPEZ Itziar;LEBONNOIS Sebastien;AO Chi;川島秀一
  • 通讯作者:
    川島秀一

TAKAGI Masahiro的其他文献

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{{ truncateString('TAKAGI Masahiro', 18)}}的其他基金

Dynamics and material circulation in the Venus upper atmosphere
金星高层大气的动力学和物质循环
  • 批准号:
    17H02961
  • 财政年份:
    2017
  • 资助金额:
    $ 9.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Study on the Venus atmospheric superrotation with a focus on the thermal tide and mean meridional circulation mechanisms
金星大气超自转热潮和平均经向环流机制研究
  • 批准号:
    25400470
  • 财政年份:
    2013
  • 资助金额:
    $ 9.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of an alternative method to animal test for evaluation of skin-irritation
开发动物试验的替代方法来评估皮肤刺激
  • 批准号:
    25630374
  • 财政年份:
    2013
  • 资助金额:
    $ 9.34万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Residues of tree harvest affect growth of planted seedlings
树木采伐残留物影响种植幼苗的生长
  • 批准号:
    21580184
  • 财政年份:
    2009
  • 资助金额:
    $ 9.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Dynamics of giant liposomes and cellular signal tranduction
巨型脂质体动力学和细胞信号转导
  • 批准号:
    20360370
  • 财政年份:
    2008
  • 资助金额:
    $ 9.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Study on the control strategy against dengue vector mosquitoes in Vietnam
越南登革热病媒蚊控制策略研究
  • 批准号:
    19256002
  • 财政年份:
    2007
  • 资助金额:
    $ 9.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Study on the Venus atmospheric superrotation induced by the thermal tides.
热潮引起的金星大气超自转研究.
  • 批准号:
    19540457
  • 财政年份:
    2007
  • 资助金额:
    $ 9.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Dynamics of protein structure alteration at single molecular level
单分子水平蛋白质结构改变的动力学
  • 批准号:
    17360395
  • 财政年份:
    2005
  • 资助金额:
    $ 9.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Evaluation on environmental factors affecting to the mosquito-borne infectious diseases in Southeast Asia
东南亚蚊媒传染病环境因素影响评价
  • 批准号:
    14256003
  • 财政年份:
    2002
  • 资助金额:
    $ 9.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Effects of environmental changes on vector mosquitoes
环境变化对媒介蚊子的影响
  • 批准号:
    11691215
  • 财政年份:
    1999
  • 资助金额:
    $ 9.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)

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Probing Amyloid Fibril Self-Assembly with Network Hamiltonian Simulations in Explicit Space
用显式空间中的网络哈密顿模拟探测淀粉样蛋白原纤维的自组装
  • 批准号:
    10715891
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    2023
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Determining How Amyloid-β Fibril Polymorphism Influences Cellular Toxicity
确定淀粉样蛋白-β原纤维多态性如何影响细胞毒性
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    10982804
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    2023
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The role of master regulator NtrC in amyloid fibril dependent pathogenic traits of Pseudomonas aeruginosa
主调节因子 NtrC 在铜绿假单胞菌淀粉样原纤维依赖性致病性状中的作用
  • 批准号:
    10990786
  • 财政年份:
    2023
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    $ 9.34万
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Combining electron and nuclear magnetic resonance to track Alzheimer's amyloid-beta oligomer-to-fibril conversion
结合电子和核磁共振来追踪阿尔茨海默病β淀粉样蛋白寡聚物到原纤维的转化
  • 批准号:
    10662904
  • 财政年份:
    2023
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The role of master regulator NtrC in amyloid fibril dependent pathogenic traits of Pseudomonas aeruginosa
主调节因子 NtrC 在铜绿假单胞菌淀粉样原纤维依赖性致病性状中的作用
  • 批准号:
    10652868
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Development, control, and functional significance of variations in collagen fibril nanostructure, with application to the creation of novel biomaterials
胶原纤维纳米结构变化的开发、控制和功能意义,及其在新型生物材料创建中的应用
  • 批准号:
    RGPIN-2020-06035
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    2022
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    Discovery Grants Program - Individual
Molecular recognition of pathological tau fibril conformations
病理性 tau 原纤维构象的分子识别
  • 批准号:
    10602405
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    2022
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Allosteric control of collagen fibril degradation by matrix metalloprotease-1
基质金属蛋白酶-1 对胶原原纤维降解的变构控制
  • 批准号:
    10402052
  • 财政年份:
    2022
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    $ 9.34万
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Allosteric control of collagen fibril degradation by matrix metalloprotease-1
基质金属蛋白酶-1 对胶原原纤维降解的变构控制
  • 批准号:
    10853496
  • 财政年份:
    2022
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    $ 9.34万
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Mechanisms of aggregation and fibril formation of amyloidogenic proteins with amyloid cofactors
淀粉样蛋白与淀粉样辅因子的聚集和原纤维形成机制
  • 批准号:
    22K06556
  • 财政年份:
    2022
  • 资助金额:
    $ 9.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
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