Mechanism of nutrient supply in the metamorphosed stage of anomurans, particularly paralithods, and its application for the resource enhancement.

camtschaticus和p.b aithodes brevipes（十足目：反常目；石蟹科：副王蟹）是北太平洋和鄂霍次克海的重要商业王蟹。本研究首次阐明了青光眼在变态阶段不采食。(1)比较了紫藻、青花和幼鱼的口器。青光骨的下颌骨，尤其是侧叶的大小减小。齿突未钙化，缺牙。上颌骨的端部和内足发育有微小的刚毛。(2)海藻前肠的刚毛减少，滤压器发育不全。没有复杂的胃磨，在第一代幼鱼发育良好。这些事实表明，喂养和食品加工在青光眼中不起作用。新孵化的动物在中肠腺的上皮细胞（吸收细胞）中有脂质储备。在幼体发育的所有阶段，饲喂人工硅藻海硅藻（thalassisira sp.）和藻黄蒿（Artemia nauplii）后，在中肠腺的中间部分观察到更多的脂质积累。青光眼早期出现的大脂滴在青光眼晚期消失。青光眼晚期，小脂滴在中肠腺表上皮细胞外缘大量分布。脂肪酸分析表明，刚孵出的黄颡鱼幼体的中性脂和极性脂含量分别为41和84 ug/干重mg，在幼体终孵期和青光期早期分别为100和110 ug/mg左右。脂质含量在青光眼中期降至60、80 ug/mg，后期降至25、50 ug/mg，首幼鱼降至15.30 ug/mg，中性脂质含量较极性脂质含量显著升高/降低。这些事实表明，在非摄食青光眼阶段，脂质被用作能量来源。在青光眼中没有观察到存在于葛根血色素中的脂肪体。这可能意味着在沿海招募期间，与葛冈的长距离迁移相比，葛冈的迁移距离相对较短。少

Paralithodes camtschaticus and P.brevipes (Decapoda : Anomura : Lithodidae : Paralithodes) are commecially important king crabs in the North Pacific Ocean and Okhotsuk Sea. The present study has elucidated, for the first time, that the glaucothoe, metamorphose stage, does not feed. (1) The mouthparts of zoea, glaucothoe and juveniles were compared. The mandibles of the glaucothe, especially the lateral lobes showed reduction in size. The gnathal processes are uncalcified, lacking denticles. The endites and endopods of the maxillae are rudimentally having minute setae. (2) The setae which presented in the foregut of zoea are reduced and the filter press is poorly developed in glaucothoe. The complex gastric mill is absent, which is well developed in the first juvenile. These facts suggest that feeding and food processing are not functional in the glaucothoe.The newly hatched zoea has lipid reserve in the epithelial cells (resorptive cells) of the midgut gland. Accumulation of lipids is … More observed in the medial portion of the midgut gland with feeding cultured diatom Thalassiosira sp. and Artemia nauplii during all zoea stages. The large lipid droplets obseved in the early glaucothoe disappeared in the late glaucothoe. Small lipid droplets distribute abundantly in the outer margin of the epithelial cells of the midgut gland tables in the late glaucothoe. Fatty acids analysis showed an increase of neutral and polar lipids contents at 41 and 84 ug/dry weight mg for the newly hatched zoea of P.camtschaticus to about 100 and 110 ug/mg at the final zoea and the early glaucothoe. The lipids content decreased to 60 and 80 ug/mg at the mid-stage glaucothoe, 25, 50 ug/mg at the late-stage, and 15.30 ug/mg at the first juvenile Neutral lipids content increased/decreased remarkably compared to polar lipids content. These facts indicate that the lipids are used as energy source during the non-feeding glaucothoe stage. The fat body, which presents in the hemocoel of the puerulus, was not observed in the glaucothoe. This may imply relatively short distance migration in glaucothoe compared to long distance migration in puerulus during coastal recruitment. Less