Study for the diagnosis of early cartilage degradation in the patient with TMJ internal derangement

在这项研究中，我们检测了关节液(SF)中的生化标记物，以检测TMJ疾病的关节内炎和早期软骨退化。SF来自22例关节内紊乱(ID)或骨关节炎(TMJ-OA)患者的25个TMJ，以及11名正常志愿者的15个无症状TMJ。用酶联免疫吸附试验测定细胞因子水平，用酶标法测定细胞内蛋白水解酶活性。伴有ID和OA的TMJ患者的SF水平较高(P<330.1.05)。[)347.7pg/100mug SF蛋白)的IL-1β水平明显高于无症状对照组(76.7()Sy.+-。[)95.3pg/100mug SF蛋白)。患有骨性关节炎的TMJ患者的SF中IL-1β水平显著升高(P<531.8)。[)379.6pg/100mug SF蛋白)和IL-6(979()SY+-.[)552pg/100mug SF蛋白)高于ID患者(IL-1β：216.7(SY+-))。[)280.1pg，IL-6：293(SY+-)。[)434pg)。在TMJ-SF(无论是正常还是疾病)中均有两种基质金属蛋白酶(MMPs)存在，其明胶溶解活性分别为92 kDa和72 kDa。还检测到相对分子质量为83 kDa和66 kDa的弱条带。Western印迹分析表明，这两种蛋白均为MMP9(83 KDa)和MMP2(66 KDa)的活性形式。IL-1β和IL-6水平较高。提示细胞因子(IL-1和IL-6)和活性形式的MMPs水平升高可能是TMJ软骨降解的潜在分解代谢标志。

In this research project, biochemical markers in the synovial fluid (SF) for detecting intra-articular inflammation and early cartilage degradation of the temporomandibular joint (TMJ) disease were examined. SF was obtained from 25 TMJs in 22 patients with internal derangement (ID) or osteoarthritis (TMJ-OA), 15 asymptomatic TMJs in 11 normal volunteers. Cytokine levels were measured by ELISA,and the proteinase activities were detected by enzymography. SF from TMJs with ID and OA showed higher (p<0.05) levels (330.1(]SY.+-。[)347.7pg/100mug of SF protein) of IL-1 beta than the asymptomatic control TMJs (76.7(]SY.+-。[)95.3pg/100mug of SF protein). SF from TMJs with OA contained significantly (p<0.05) higher levels of IL-1beta (531.8(]SY.+-。[)379.6pg/100mug of SF protein) and IL-6 (979(]SY.+-。[)552pg/100mug of SF protein) than those with ID (IL-1beta : 216.7(]SY.+-。[)280.1pg, IL-6 : 293(]SY.+-。[)434pg). Two matrix metalloproteinases (MMPs) with gelatinolytic activities at 92kDa and 72kDa were constitutively detected in both the TMJ-SF (either normal or disease). Also detected were weak bands with molecular weight of 83kDa and 66kDa. Western blot analysis delineated that these were active forms of MMP-9 (83kDa) and MMP-2 (66kDa). The bands were also detected in TMJ with OA which showed high levels of IL-1beta and IL-6.These findings suggest that concomitant increases, in the levels of cytokines (IL-1 and IL-6) and active forms of MMPs, would be potential catabolic markers for cartilage degradation of the TMJ.