魚類のトリアシルグリセロール代謝の制御機構に関する研究

利用下一代测序技术(NGS)对青蛙肌肉和肝脏中蛋白质和糖原/葡萄糖代谢相关基因的表达模式进行了分析，并对NGS数据进行了统计分析，揭示了青蛙肌肉和肝脏之间完全不同的代谢反应。在禁食4天期间，肌肉似乎更喜欢消耗代谢过程所需的蛋白质和糖原，而肝脏似乎消耗蛋白质、糖原和TAG储备。禁食8天后，肌肉中TAG代谢活性增强，糖原消耗减少。相反，肝脏会减少TAG、蛋白质和糖原储备的支出。再投喂会诱导青竹肌肉中TAG和碳水化合物资源的代谢水平和蛋白质的恢复。在代谢过程中，脂蛋白脂酶(LPL)调控的TAG转运的变化与两种组织中TAG水平的变化密切相关。因此，为进一步研究LPL在Tag积累中的作用，构建了青冈LPL表达载体。不幸的是，显微注射构建的质粒的斑马鱼胚胎没有显示绿色荧光。为了研究构建的LPL表达载体的质量和效率，我们用ZF4细胞进行了转染实验。

To investigate the potential correlations of metabolisms among the internal energy sources, the analyses of the expression patterns of the genes involved in protein and glycogen/glucose metabolisms were further performed using next generation sequencing (NGS).Statistical analyses of the NGS data revealed complete diverse metabolic responses between muscle and liver of medaka. During 4 days of fasting, muscle appears to prefer to consume protein and glycogen for the energy required by the metabolic processes, whereas liver appears to expense protein, glycogen and TAG reserves. During 8 days of fasting, the TAG metabolic activity would be induced in the muscle, while the consumption of glycogen would be reduced. In contrast, liver would reduce the expenditure of TAG, protein and glycogen reserves. Re-feeding would induce the metabolic levels of TAG and carbohydrate resources and restoration of protein in medaka muscle. In contrast, the restoration of TAGs and expenditure of glycogens would occur in the livers of re-fed medaka.Among the metabolic processes, the variation of TAG transport regulated by lipoprotein lipase (LPL) appears to be closely related to those of TAG levels in both tissues. Therefore, the construction of a medaka LPL expression vector was performed to further investigate the function of LPL on TAG accumulation. Unfortunately, the zebrafish embryos microinjected with constructed plasmid showed no green fluorescence. To investigate the quality and efficiency of constructed LPL expression vector, transfection will be performed with ZF4 cell line.