Enhancing Food Testing Capabilities at the WVDA through Discipline A: Microbiology Analytical Track 5: Microbiology Capability / Capacity Development

2020-2021财年实验室灵活资助模式 西弗吉尼亚州农业部提案 PAR-20-105： 不允许进行LFFM(U19)临床试验 学科A：微生物学 分析轨道4：全基因组测序 摘要： 全基因组测序是决定碱基顺序的实验室程序 (A、T、G和C)在一个过程中在生物体的基因组中。小片段DNA 标签或条形码被添加到DNA上，以识别剪切的DNA片段 属于哪种细菌。来自多个细菌的条形码DNA组合在一起， 放入整个基因组测序仪。测序仪识别组成的碱基 每个细菌序列，并使用条形码来跟踪哪些碱基属于 哪种细菌。计算机分析工具可以用来比较细菌序列 并找出差异。差异的数量可以说明两者之间的密切联系 细菌是，以及它们是同一疫情的一部分的可能性有多大。 全基因组测序是获得高水平信息的一种快速且负担得起的方式 只用一次测试就能了解细菌。将此级别的技术添加到 西弗吉尼亚州农业部的微生物实验室将允许 部门在实时设置中获取细菌基因组数据并协助更快地 全球范围内的疫情检测。 学科A的目的：微生物学；分析轨道4：全基因组 测序是为了加强西弗吉尼亚州内的GenomeTrakr网络 农业部监管和环境事务司通过捕获 FDA非临床病原体目前和正在演变的基因组多样性- 人类或动物食品、环境和水样中的相关领域。这个 通过此分析轨道生成的测序数据将公开存储 国家生物技术信息中心(NCBI)的数据库，并将 由研究人员和公共卫生官员访问以进行实时比较和 分析将对食源性疾病暴发调查产生积极影响并减少 食源性疾病和死亡。

Laboratory Flexible Funding Model FY2020-2021 West Virginia Department of Agriculture Proposal PAR-20-105: LFFM (U19) Clinical Trials Not Allowed Discipline A: Microbiology Analytical Track 4: Whole Genome Sequencing Abstract: Whole genome sequencing is a laboratory procedure that determines the order of bases (A, T, G, and C) in the genome of an organism in one process. Small pieces of DNA tags, or bar codes, are added to the DNA to identify which piece of sheared DNA belongs to which bacteria. The bar-coded DNA from multiple bacteria are combined and put in the whole genome sequencer. The sequencer identifies the bases that make up each bacterial sequence and uses the bar code to keep track of which bases belong to which bacteria. Computer analysis tools can be used to compare bacterial sequences and identify differences. The number of differences can illustrate how closely related the bacteria are, and how likely it is that they are part of the same outbreak. Whole genome sequencing is a fast and affordable way to obtain high-level information about the bacteria using just one test. Adding this level of technology within the Microbiology laboratory at West Virginia Department of Agriculture will allow the department to obtain bacterial genomic data in a real-time setting and assist in faster outbreak detection on a global scale. The purpose of Discipline A: Microbiology; Analytical Track 4: Whole Genome Sequencing is to enhance the GenomeTrakr network within the West Virginia Department of Agriculture Regulatory and Environmental Affairs Division by capturing the current and evolving genomic diversity of non-clinical pathogens across FDA- relevant areas in human or animal food, environmental, and water samples. The sequencing data generated through this analytical track will be housed in public databases at the National Center for Biotechnology Information (NCBI) and will be accessed by researchers and public health officials for real time comparison and analysis that will positively impact foodborne illness outbreak investigations and reduce foodborne illnesses and death.