Action and interaction of ionotropic and metabotropic neurotransmission

摘要 本文是R37 NS046579-16《行动与互动》的行政多样性补充申请 支持Rochinelle科学训练和发育的离子和代谢神经传递 东默。董默女士是埃默里大学的学士学位持有者，目前正在攻读一项 哈佛大学攻读学士学位后研究学者课程，为博士研究生课程做准备 神经科学，目标是在2021年秋季之前注册一个项目。作为一个黑人女性，她是美国人。 永久居民目前正在结束她作为喀麦隆美国公民的入籍，她是 在生物医学研究中代表性不足的一个民族。 东默在实验室的研究将追求与父母拨款相关的两个目标，这两个目标都是为了更好地 了解代谢性神经传递到非神经性小胶质细胞的机制。在第一个目标中，她说。 董默将开发一种成像分析方法，通过大体变化来评估体外脑片中小胶质细胞的激活 形态学。这项测试将使我们能够测试小胶质细胞对多种神经递质的激活，最值得注意的是 乙酰胆碱和GABA，已知的小胶质细胞具有代谢性受体。在第二个目标中， 董默女士将测量小胶质细胞激活对细胞内钙离子和蛋白激酶活性的影响 A(PKA)，两个中心性的细胞内信号分子。她将测试钙离子和PKA活性，以响应 已知的小胶质细胞激活剂，以及GABA和乙酰胆碱，它们对小胶质细胞激活的净作用 将在她的第一个目标中下定决心。在执行这项研究计划期间，她将在所有 科学和技术方面的研究。我们设计了一个全面的指导和培训 东默女士的计划包括实验室经验，与PI的定期一对一会议，以及一名高级博士后， 会议报告，所有这些都是除了她参加哈佛研究学者倡议之外的事情，该倡议 包括课程、研讨会、辅导、GRE考试准备和研究生院申请指导。这些 研究和职业发展计划将为董默女士在研究领域取得成功的学术生涯做好准备 神经科学。

Abstract This is an application for an administrative diversity supplement to R37 NS046579-16, “Action and interaction of ionotropic and metabotropic neurotransmission” to support the scientific training and development of Rochinelle Dongmo. Ms. Dongmo is a baccalaureate degree holder from Emory University who is currently enrolled in a post-baccalaureate research scholars program at Harvard to prepare for Ph.D. graduate programs in neuroscience, with the goal of enrolling in a program by the Fall of 2021. As a black woman who is a U.S. permanent resident currently finishing her naturalization as an American citizen from Cameroon, she is part of an ethnic group underrepresented in biomedical research. Ms. Dongmo's research in the lab will pursue two aims related to the parent grant, both of them seeking to better understand the mechanism of metabotropic neurotransmission to non-neuronal microglia. In the first aim, Ms. Dongmo will develop an imaging assay to assess microglia activation in ex vivo brain slices via changes in gross morphology. This assay will allow us to test the activation of microglia to multiple neurotransmitters, most notably acetylcholine and GABA, for which microglia are known to possess metabotropic receptors. In the second aim, Ms. Dongmo will measure the effects of microglia activation on the intracellular activity of Ca2+ and protein kinase A (PKA), two central intracellular signaling molecules. She will test the Ca2+ and PKA activity in response to both known activators of microglia, as well as to GABA and acetylcholine, whose net effect on microglia activation will be determined in her first aim. She will be mentored during the execution of this research plan on both all scientific and technical aspects of the research. We have designed a comprehensive mentorship and training plan for Ms. Dongmo including lab experience, regular one-on-one meetings with the PI and a senior post-doc, conference presentations, all in addition to her participation in the Harvard Research Scholars Initiative, which includes classes, workshops, tutoring, GRE test preparation, and graduate school application guidance. These research and career development plans will prepare Ms. Dongmo for a successful academic career in research neuroscience.