Cell Biology, Pathology, and Imaging Core

项目总结/摘要 生物医学研究核心协同工作，以加强研究和流动的双向增强， 数据细胞生物学、病理学和成像核心（核心C）在表型分析中占有核心和关键的地位 生理学核心（Physiology Core）核心C将在以下几个领域为肾脏研究者服务：1.病理 2.细胞生物学3.磁共振成像（MRI）。对于病理学，我们提供基础设施，设备， 服务和教育的所有标准技术，但我们仍然灵活的创新和新颖的程序， 需求出现了。对于细胞生物学，我们提供培训研究人员在固定和活细胞中进行共聚焦成像 并允许他们使用最先进的显微镜设备。对于整体动物成像，我们提供标准MR- 基于解剖学的成像，解剖学和心脏功能的心脏成像，以及各种肾功能 MRI测量许多生理和病理生理参数。我们在三点提供援助 这取决于实现科学终点所需的性质。 对于高标准的既定程序，它是作为一种服务提供的，其中调查人员支付一个小的非- 利润成本回收费和结果在双方商定的时间框架内交付。例如切片和H&E 渍.核心将被引用，但核心主任不会是任何结果手稿的共同作者。不到 由于使用频率较高和技术性较强，将以协作方式提供援助。显然， 定义的可交付成果，不收取任何费用，但预期是共同作者。例如，功能性MRI测量肾血 流量、氧合和水的表观扩散系数（纤维化/细胞浸润的替代物）。最后，研究 可以作为一种探索，调查人员与核心董事合作，发现新的 领土例如光谱计算机断层摄影。 细胞生物学、病理学和成像核心仍然是奥布莱恩表型分析的基石之一 中心除了过渡性的“主力”化验外，我们还不断开拓新的领域， 技术.核心对奥布莱恩中心的使命做出了重大贡献， 肾脏发育和遗传学、生理学和病理生理学以及慢性肾脏疾病的主题， 其并发症，长期目标是改善肾脏疾病的诊断和治疗。

PROJECT SUMMARY/ABSTRACT The Biomedical Research Core works in synergy to enhance bidirectional enhancement of research and flow of data. The Cell Biology, Pathology, and Imaging Core (Core C) holds a central and critical place in phenotyping in conjunction of the Physiology Core (Core). Core C will serve renal investigators in several areas: 1. Pathology. 2. Cell Biology. 3. Magnetic resonance imaging (MRI). For Pathology, we provide infrastructure, equipment, service, and education on all standard techniques but we remain flexible for innovative and novel procedures as the need arises. For Cell Biology, we offer to train investigators to perform confocal imaging in fixed and live cells and allow them access to a state-of-the art microscopy suite. For whole animal Imaging, we offer standard MR- based anatomic imaging, cardiac imaging for anatomy and cardiac function, and a wide variety of renal functional MRI to measure a number of physiology and pathophysiologic parameters. We offer our assistance at three levels depending on the nature of what is required to achieve the scientific endpoint. For the highly standard established procedure, it is offered as a service where the investigators pay a small non- profit cost recovery fee and the results are delivered in mutually agreed time frames. e.g. sectioning and H&E stains. The Core will be cited but Core Directors will not be co-authors on any resultant manuscripts. For less frequently used and more technical assays, the assistance will be provide as a collaboration. There will be clearly defined deliverables with no charge but joint authorship is expected. e.g. functional MRI to measure renal blood flow, oxygenation, and apparent diffusion coefficient of water (surrogate of fibrosis/cell infiltrate). Finally, studies can be performed as an exploration where the investigators work with the Core Directors to discovery new territories. e.g. spectral computed tomography. The Cell Biology, Pathology, and Imaging Core remains one of cornerstones of phenotyping in the O'Brien Center. In addition to transitional “workhorse” assays, we continuously explore new territories to advance the technology. The Core contributes significantly to the mission of the O'Brien Center to conduct research in the themes of kidney development and genetics, physiology and pathophysiology, and chronic kidney disease and its complications, with the long term goal of improving diagnosis and treatment of kidney diseases.