Wadsworth Center's Radiochemical, Microbiological and Chemical Testing for Food Defense and Capability Development (Discipline A: Microbiology, Analytical Track 1: Food Defense and Maintenance)

摘要 该项目的目标是提高利用蕨类和植物的微生物威胁物质的分析能力。 提高实验室食品防御任务和疫情应对能力的其他方法。作为一名 具有生物安全级别2(BSL-2)和生物安全级别3(BSL-3)的第1级选择代理注册机构 实验室，我们经验丰富的工作人员每月能够进行500多次分析 生物治疗剂和毒素。 沃兹沃斯中心在纽约的食品检测中发挥着积极的作用。我们的实验室与食品有关 疫情调查、常规食品监测以及其他州和地区的确认性检测资源 参与食品监管的联邦机构。我们对其进行识别和表征 从人类样本中分离出的微生物以及相关的相关食物来源 作为经典的微生物学方法，内部开发了RT-PCR、DNA测序、分子菌株和 血清分型和DNA指纹分析。除了蕨类和LRN，沃兹沃斯中心也参与了 在疾控中心资助的新兴传染病项目中，包括FoodNet、PulseNet和National 阻力监测系统。沃兹沃斯中心与纽约卫生局密切合作 传染病控制和食品保护局以及其他州和联邦合作伙伴 包括农业和市场部以及FDA和美国农业部，以协助提供 食品中精选微生物和毒素的分离鉴定及及时、协调 应对与食物有关的疫情。 生物防御实验室的工作人员参加了由费恩赞助的培训，以及开发和 作为主题专家提供了几次培训。最近由我们的成员领导的培训 实验室是一门MALDI-TOF MS应用课程，用于生物制剂和毒素。MALDI-TOF MS 许多州和联邦农业和公共卫生实验室正在采用这种方法。我们的 实验室目前正在协助其他州和联邦机构验证和实施这些新方法 他们的实验室以及参加每月一次的肉毒梭菌协调电话会议 FDA、USDA和CDC的成员，我们是唯一的公共卫生实验室成员。专业知识 已经允许开发和验证多种新方法，这些方法目前 用于筛选食品中的高风险病原体和毒素。 该项目的预期结果是确保FDA分发的食品商品可以安全消费 为普通公众提供服务，并为蕨类提供更强的能力和能力。此外，预计 在大型聚会和活动期间，食品供应将是安全的，不会受到故意污染，例如 政治会议、就职典礼和超级碗。

ABSTRACT The goal of this project is to enhance the ability to analyze for microbiological threat agents utilizing FERN and other methods to improve laboratory capacities for food defense assignments and outbreak response. As a Tier 1 Select Agent registered facility with both Biosafety Level 2 (BSL-2) and Biosafety Level 3 (BSL-3) laboratories, our highly experienced staff is capable of performing more than 500 analyses per month for biothreat agents and toxins. The Wadsworth Center plays an active role in food testing in NYS. Our laboratory is involved with food outbreak investigations, routine food surveillance, and a resource for confirmatory testing for other state and federal agencies involved in the regulation of food. We perform identification and characterization on organisms isolated from human specimens as well as associated implicated food sources using methods such as classical microbiological methods, in-house developed rtPCR, DNA sequencing, molecular strain and serotyping, and DNA fingerprinting. In addition to the FERN and LRN, the Wadsworth Center also participates in the Emerging Infectious Program funded by the CDC which includes FoodNet, PulseNet, and the National Resistance Monitoring System. The Wadsworth Center works very closely with the NYS DOH Bureau of Communicable Disease Control and Bureau of Food Protection as well as other state and federal partners including Department of Agriculture and Markets and the FDA and USDA to assist in providing expertise in the isolation and identification of select agent microbes and toxins in food products and a timely, coordinated response to food related outbreaks. Staff in the Biodefense Laboratory have attended FERN sponsored trainings as well as developed and delivered several trainings as subject matter experts. The most recent training that was led by members of our laboratory was a MALDI-TOF MS applications course for biothreat agents and toxins. The MALDI-TOF MS methods are being adopted by many state and federal agricultural and public health laboratories. Our laboratory is currently assisting other state and federal agencies validate and implement these new methods in their laboratories as well as participate in a monthly Clostridium botulinum harmonization conference call with members of the FDA, USDA, and CDC where we are the only public health laboratory member. The expertise of our laboratorians has allowed for the development and validation of multiple new methods that are currently being used to screen food commodities for high risk pathogens and toxins. The expected outcome of this project is to ensure that FDA distributed food commodities are safe to consume for the general public and to provide enhanced capabiity and capacity for FERN. Additionally, it is expected that the food supply will be safe from intentional contamination during large gatherings and events, such as political conventions, inaugurations, and the Super Bowl.