Cryo Transmission Electron Microscope for Cryo-EM Sample Optimization

项目摘要/摘要 低温电子显微镜(Cryo-EM)预示着结构生物学领域的一场分辨率革命，它已经使 有可能为仍然顽固的目标确定高分辨率结构 几十年来的结晶。康奈尔大学通过购买高端电子产品来投资于这一未来。 显微镜下，北极带着K3，为研究人员提供了一个重要的宝贵工具，用来揭示原子 他们冷冻的生物样本的详细信息。不幸的是，我们计划失去我们敬业的低端 屏幕显微镜，Morgagni，2021年的某个时候，或者可能是2022年。在分析我们当前的工作流程和 康奈尔大学结构生物界的需求，我们得出的结论是， Morgagni是一台F200Ci显微镜，既能收集负染数据，也能进行低温电子显微镜成像。 获得这样的显微镜将提高康奈尔大学科学家和美国国立卫生研究院资助的 研究人员的研究计划，最大限度地利用我们最高分辨率和最畅销的产品 配备了对高分辨率数据收集至关重要的附件的显微镜：a K3 Direct 探测器和生物量子能量过滤器。这个应用程序申请资金购买一个多功能的低温电子 理想的显微镜能够进行负染成像、低温样品筛选和微电子衍射 (MicroED)。它将为美国国立卫生研究院资助的康奈尔大学五个系的教职员工提供服务，所有这些系都投资于追求这一结构 与人类健康有关的生物医学相关和重要生物系统，包括膜 受体，膜转运，参与基因组组织的核酸复合体，以及病毒结构。

PROJECT SUMMARY/ABSTRACT Cryo-electron microscopy (Cryo-EM) has heralded a resolution revolution in structural biology that has made it possible for high-resolution structures to be determined for targets that have remained recalcitrant to crystallization for decades. Cornell has invested in this future with the purchase of a high-end electron microscope, the Arctica with a K3, allowing researchers an important valuable tool with which to reveal the atomic details of their cryo-frozen biological samples. Unfortunately, we are scheduled to lose our dedicated low-end screening microscope, the Morgagni, sometime in 2021 or possibly 2022. In analyzing our current workflow and needs of the structural biology community at Cornell, we have concluded that the optimal replacement for the Morgagni is a F200Ci microscope capable of both negative stain data collection and cryo-EM imaging. Acquiring such a microscope will improve overall throughput for Cornell scientists and the NIH-funded investigators’ research programs, maximizing the utility of our highest-resolution and most in-demand microscopes which are equipped with accessories crucial for high-resolution data collection: a the K3 direct detector and a BioQuantum Energy Filter. This application requests funds to purchase a versatile cryo-electron microscope ideally capable of negative stain imaging, cryo-sample screening, and micro-electron diffraction (microED). It will serve NIH-funded faculty in five departments across Cornell, all invested in pursing the structure of biomedically relevant and important biological systems related to human health, including membrane receptors, membrane trafficking, nucleic-acid complexes involved in genome organization, and viral structure.