Acquisition of a Beckman-Coulter Optima Analytical Ultracentrifuge

收购Beckman-Coulter Optima分析超灵敏度仪 项目概要/摘要： 该提案详细说明了Beckman-Coulter Optima分析超离心泵的资金申请， 波长紫外-可见吸收和瑞利干涉检测光学器件，以及必要的附件。 该仪器将安装在约翰逊研究基金会结构生物学和生物物理学核心 （JRFSBBC）由Perelman生物化学和生物物理系监督的设施 宾夕法尼亚大学医学院。该仪器将支持NIH资助的研究， 宾夕法尼亚大学和大费城地区其他研究机构的研究人员， 与NIH和NSF资助的来自美国各地的研究人员合作， 提供，并培训研究生和博士后学员。这一新工具将取代 约25年历史的Beckman XL-A分析超滤仪（AUC），目前为核心服务。新 仪器代表了分析超浓缩的重要进展，并使应用 出现了利用多波长数据收集的方法;这在旧的情况下目前是不可能的 仪器这些技术进步包括改进的波长再现性，大大改善的信号- 噪声和更快的扫描速率，这将共同提供上级精度的研究， 溶液中大分子缔合和形状的热力学。这些研究项目将 先进的是范围广泛，所有的高影响，包括艾滋病毒药物的发现，癌症，抗生素耐药性， 整合膜蛋白药物靶点、神经退行性疾病和染色质结构。的 所寻求的技术相对较新，在全国范围内的学术环境中几乎没有这样的仪器 （自2017年初），预计将影响大量的内部和外部用户。仪器将 由JRFSBBC经验丰富的团队管理，该团队成立于2016年， 强大的研究环境。

Acquisition of a Beckman-Coulter Optima Analytical Ultracentrifuge Project Summary/Abstract: This proposal details a funding request for a Beckman-Coulter Optima analytical ultracentrifuge with multi- wavelength UV-Visible absorption and Rayleigh interference detection optics, with necessary accessories. The instrument will be installed in the Johnson Research Foundation Structural Biology and Biophysics Core (JRFSBBC) Facility that is overseen by the Department of Biochemistry and Biophysics at the Perelman School of Medicine of the University of Pennsylvania. The instrument will support NIH-funded research by investigators at Penn and other research institutions in the Greater Philadelphia region, collaborative research with NIH and NSF-funded researchers from across the US where similar technology and expertise is not available, and the training of graduate students and postdoctoral trainees. This new instrument will replace a ~25-year-old Beckman XL-A analytical ultracentrifuge (AUC) that currently serves the core. The new instrument represents an important advance in analytical ultracentrifugation and enables the application of arising methods that leverage multiwavelength data collection; this is currently not possible with the older instrument. These technical advances include improved wavelength reproducibility, vastly improved signal-to- noise, and faster scanning rates, which together will provide superior precision in the study of the thermodynamics of macromolecular associations and shape in solution. The research projects that will be advanced are broad in scope and all high-impact, including HIV drug discovery, cancer, antibiotic resistance, integral membrane protein drug targets, neurodegenerative disorders, and chromatin structure. The technology sought is relatively new, with few such instruments available in the academic setting nationwide (since early 2017) and is expected to impact a large group of intra- and extramural users. The instrument will be managed by the very experienced team at the JRFSBBC, which was established in 2016, within an already large and robust research environment.