ShEEP Request for a SpectraMax M Series Multi-Mode Microplate Reader

在本申请中，我们要求ORD共享设备评估计划（ShEEP）提供资金， 多模式酶标仪。该仪器将作为共享资源，存放在研究处 奥马哈退伍军人医疗中心具体来说，我们寻求获得光谱最大M系列多模式微孔板 读者SpectraMax M系列可用于比色皿和微孔板阅读， 荧光和发光。这一重要设备将提供最先进的能力， 目前可在我们的VAMC校园。它将取代我们目前的酶标仪，这是10岁， 需要大量昂贵的维修有了这种新仪器，就不需要使用昂贵的过滤器， 优化检测水平和背景。光学系统使用两个扫描单色器，使得 用户可以确定最佳的激发和发射设置，从而使检测性能与 专用的单模读卡器。此外，Spectra Max M系列还提供测量 使用获得专利的PathCheck®传感器，测量样品深度（光程长度），无温度依赖性 技术.使用SoftMax Pro软件，PathCheck传感器可自动标准化微孔吸光度。 这消除了对标准曲线和具有已知吸光度特性的化合物的需要， 用户可以直接从吸光度计算浓度。酶标仪将广泛用于 奥马哈VAMC研究服务部的调查员该仪器将使新的能力， 奥马哈VAMC和我们在内布拉斯加大学医学中心（UNMC）的附属机构的研究人员。 收购Spectramax将帮助我们创建该医疗中心目前无法提供的新功能。 SpectraMax M系列将有助于加速我们的研究。具体来说，我们正在研究退伍军人相关， 临床前疾病模型，包括肝脏疾病，糖尿病和肺部疾病，希望推进 支持VA使命的治疗干预。

In this application, we request funds from the ORD Shared Equipment Evaluation Program (ShEEP) to purchase a Multi-Mode Microplate Reader. This instrument will be a shared resource to be housed in the Research Service of the Omaha VA Medical Center. Specifically, we seek to obtain a Spectra Max M Series Multi-Mode Microplate Reader. The SpectraMax M series allows for both cuvette and microplate reading and detection by UV, visible, fluorescence and luminescence. This important piece of equipment will provide state-of-the art capabilities not currently available on our VAMC campus. It will replace our current microplate reader, which is 10 years old and needs extensive and expensive repairs. With this new instrument, there is no need to utilize expensive filters to optimize detection levels and background. The optical systems use two scanning monochromators, so that the user can determine optimal excitation and emission settings, resulting in assay performance similar to that of dedicated single-mode readers. Additionally, the Spectra Max M Series offers the capability of measuring the depth (optical pathlength) of samples with no temperature dependency, using the patented PathCheck® Sensor technology. With SoftMax Pro Software, the PathCheck Sensor automatically normalizes the well absorbance. This eliminates the need for standard curves, and for compounds with known absorbance properties, enabling users to calculate concentrations directly from absorbance. The microplate reader will be extensively used by our investigators in the Research Service of the Omaha VAMC. The instrument will enable new capabilities for researchers at the Omaha VAMC and our affiliates at the University of Nebraska Medical Center (UNMC). Acquisition of the Spectramax will help us create new capabilities not currently available at this Medical Center. SpectraMax M Series will help to accelerate our research. Specifically, we are examining Veteran-relevant, preclinical disease models, including liver disease, diabetes and pulmonary disease in hopes of advancing the therapeutic interventions that will support the VA mission.