Transgenic & Knock-out Mouse

总结 很明显，未来几十年糖尿病研究的进展将在很大程度上取决于 利用小鼠作为实验模型，研究基础和临床相关问题， 糖尿病研究人类和小鼠基因组之间的高度保守性使得 使用基因改变小鼠技术来创建人类糖尿病、内分泌病理学和 糖尿病并发症非常有用。转基因和敲除小鼠核心（TKMC）提供了 加州大学洛杉矶分校和加州大学圣地亚哥分校的研究人员在小鼠中进行了广泛的遗传操作，包括靶向 使用CRISPR/Cas9的缺失、插入和突变，转基因，同源重组， 胚胎干细胞（ES细胞），从ES细胞创造嵌合小鼠，以及最前沿的 在小鼠中进行反向遗传学的方法。转基因、敲除和CRISPR小鼠模型 是利用最先进的方法，包括条件性Tet-inducible和他莫昔芬， 可诱导的转基因，使用Cre-LoxP和Flp重组酶的组织特异性和条件性敲除， 重组介导的盒交换（RMCE），BAC转基因，BAC-Trap，RiboTag，和其他 专业技术。这个长期存在的核心是一个杰出的例子， 技术、训练有素的专职人员、专门建造的设施和昂贵的设备， 研究人员不能合理地期望在个人基础上开发它们。 主要目标是： 1.为糖尿病及其并发症的研究创造创新和重要的小鼠模型。 2.为DRC成员消除最先进的小鼠遗传方法的障碍。 3.提供优秀、可靠、高质量的小鼠胚胎学和遗传学服务。 4.推进小鼠基因组的遗传操作技术。 这种转基因和敲除小鼠核心的可用性与其他DRC核心协调，将 继续使我们的成员能够在小鼠中进行多功能，尖端，反向遗传研究， 一系列多学科的最先进的技术

Summary It is clear that the progress of diabetes research during the coming decades will depend heavily upon the ability to utilize the mouse as an experimental model to investigate both basic and clinically relevant questions in diabetes research. The strong conservation between the genomes of humans and mice makes the approach of using genetic alteration mouse technology to create models for human diabetes, endocrine pathologies, and diabetes complications extremely useful. The Transgenic and Knock-out Mouse Core (TKMC) provides investigators at UCLA and UCSD with a wide array of genetic manipulations in the mouse including targeted deletions, insertions, and mutations using CRISPR/Cas9, transgenic genes, homologous recombination in embryonic stem cells (ES cells), creation of chimeric mice from ES cells, and the most cutting-edge approaches to performing reverse genetics in the mouse. Transgenic, knock-out, and CRISPR mouse models are created that utilize the most advanced approaches including conditional Tet-inducible and tamoxifen- inducible transgenes, tissue-specific and conditional knock-outs using Cre-LoxP and Flp recombinases and recombination–mediated cassette exchange (RMCE), BAC transgenics, BAC-Trap, RiboTag, and other specialized technologies. This long-standing Core is an outstanding example of how extraordinarily specialized techniques, highly trained dedicated personnel, specially constructed facilities, and expensive equipment can be accessed by researchers who could not reasonably expect to develop them on an individual basis. Key objectives are: 1. To create innovative and important mouse models for studies of diabetes and its complications. 2. To eliminate barriers to the most cutting-edge mouse genetic approaches for the DRC membership. 3. To provide outstanding, reliable, and high quality mouse embryology and genetic services. 4. To advance the technology of genetic manipulation of the mouse genome. The availability of this Transgenic and Knock-out Mouse Core in coordination with the other DRC Cores, will continue to enable our members to conduct versatile, cutting-edge, reverse genetic research in the mouse with a battery of multidisciplinary, state-of-the-art techniques.