Flow Cytometry Core

流式细胞术核心 摘要 流式细胞仪核心将提供干细胞分离、细胞分选和细胞表型分析方面的专业知识 分析.《核心》将由洛伦·法斯特博士和马克·杜纳先生共同执导。杜纳先生是一位经验丰富的 流式细胞仪与神经和造血干细胞的分选和分析，并将监督日常 操作包括细胞分选和分析。法斯特博士将担任研究人员的顾问和资源 用于实验设计、数据解释和手稿准备。在第一阶段， 创建了一个五激光细胞分选机，这是独一无二的罗得岛研究界。此核心具有 Influx高速细胞分选仪（BD Biosciences）。该分选机能够进行干细胞分离， 单细胞沉积和同时的六向分选，并被容纳在生物安全柜中。该芯 住在一个新建的专用套房里。该设施配备有LSRII流式细胞仪（BD Biosciences）与FACS Diva软件进行细胞周期分析和常规流式细胞术分析。调查人员 将与核心一起工作，准备他们的细胞群进行分类或分析。核心将服务于所有人 在这个COBRE项目中免费进行分离和分析工作。非COBRE 研究人员正在以服务收费的方式使用核心，但COBRE项目将被授予 要务核心仍然致力于开发新的分离技术和提高 已知的神经和造血干细胞群。

Flow Cytometry Core Abstract The Flow Cytometry Core will provide expertise in stem cell separations, cell sorting and cell phenotyping analysis. The Core will be co-directed by Dr. Loren Fast and Mr. Mark Dooner. Mr. Dooner is an experienced flow cytometrist with sorting and analysis of neural and hematopoietic stem cells, and will oversee the daily operation including cell sorting and analysis. Dr. Fast will serve as an advisor and resource to the investigators for experimental design, data interpretation and manuscript preparation. During Phase I, instrument upgrades created a five laser cell sorter that is unique in the Rhode Island research community. This Core features an Influx high speed cell sorter (BD Biosciences). This sorter is capable of carrying out stem cell separations, single cell deposition, and simultaneous six-way sorting and is housed within a biosafety cabinet. This Core is housed in a newly constructed and dedicated suite. The facility is equipped with a LSRII flow cytometer (BD Biosciences) with FACS Diva software for cell cycle analysis and routine flow cytometry analysis. Investigators will work with the Core and prepare their cell populations for sorting or analysis. The Core will serve all projects in this COBRE carrying out both separations and analysis work free of charge. Non-COBRE investigators are making use of the Core on a fee for service basis however COBRE Projects will be given priority. The Core remains dedicated to the development of new separation techniques and the enhancement of known neural and hematopoietic stem cell populations.