Flow Cytometry Core

流式细胞术核心

• 批准号: 10630389
• 负责人: MARK S DOONER
• 金额: $ 21.32万
• 依托单位: RHODE ISLAND HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-01 至 2028-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10630389
• 关键词: Advisory Services; Aging; Area; Basic Science; Biomedical Research; Buffers; Cell Separation; Cells; Centers of Research Excellence; Clinical; Clinical Research; Collaborations; Communities; Core Facility; Cytometry; Data Collection; Data Storage and Retrieval; Dedications; Discipline; Experimental Designs; Flow Cytometry; Goals; Growth; Health Personnel; Hospitals; Human Resources; Inductively Coupled Plasma Mass Spectrometry; Institution; Lasers; Longevity; Mass Spectrum Analysis; Phase; Phenotype; Pilot Projects; Population; Positioning Attribute; Property; Research; Research Activity; Research Personnel; Research Project Grants; Resources; Rhode Island; Role; Services; Sodium Chloride; Solid; Source; Suspensions; System; Techniques; Technology; Tissues; Universities; instrumentation; laser cell sorter; particle; programs; single cell analysis; stem cells; success; time of flight mass spectrometry; tool

Flow Cytometry Core Abstract The Flow Cytometry Core was initially formed to fulfill the flow cytometry needs of the projects and pilot projects of the original COBRE Phase I application. The Core provided expertise in stem cell and rare cell separations, cell sorting and cell phenotyping analysis, by making available, state-of-the-art instrumentation for the flow cytometry needs of the COBRE investigators as well as the clinical and basic research community in Rhode Island. Today, the Flow Cytometry Core continues to fulfill this critical role and supports a multitude of research programs, projects and diverse research areas at Rhode Island Hospital and different institutions in the state of Rhode Island. It remains heavily utilized, partially due to the strong support and success of the COBRE’s here in Rhode Island but also the strategic importance and basic relevance that flow cytometry brings to the research community at large. This is the only research flow cytometry core facility at Rhode Island Hospital and the only research flow cytometry core facility that offers access to a 5-laser sorter, 4-laser analyzer and a mass cytometer in the state. Flow cytometry remains an extremely useful tool for researchers over many different research disciplines. The institution has been generous with its administrative and fiscal support of the Flow Cytometry Core. Notably, this year Rhode Island Hospital has purchased and installed, a Fluidigm Helios CyTOF system, that is now available through the COBRE Flow Cytometry Core. This CyTOF platform will be utilized by clinical and basic researchers in the State of Rhode Island and shows a high level of support and dedication to the success of the proposed COBRE Flow Cytometry Core. With the new state-of-the-art instrumentation and Core expansion plan directed towards expanding inclusion of clinical research projects, our Core is well positioned to continue its growth and achieve long-term financial sustainability.

流式细胞术核心 摘要 流式细胞术核心最初是为了满足项目和试点项目的流式细胞术需求而形成的 最初的COBRE第一阶段应用。核心提供干细胞和稀有细胞分离方面的专业知识， 细胞分选和细胞表型分析，通过提供最先进的流动仪器， COBRE研究人员以及罗德岛临床和基础研究社区的细胞计数需求 岛今天，流式细胞仪核心继续履行这一关键作用，并支持大量的研究 计划，项目和不同的研究领域在罗得岛医院和不同的机构在国家 罗得岛。它仍然大量使用，部分原因是强大的支持和成功的眼镜蛇在这里 在罗得岛，还强调了流式细胞术给研究带来的战略重要性和基本相关性 整个社区。这是罗得岛医院唯一的研究流式细胞术核心设施， 研究流式细胞术核心设施，提供5激光分选仪、4激光分析仪和质谱仪 在状态流式细胞术仍然是一个非常有用的工具，为研究人员在许多不同的研究 学科该机构一直慷慨地提供流式细胞术的行政和财政支持 核心值得注意的是，今年罗得岛医院购买并安装了Fluidigm Helios CyTOF系统， 现在可以通过COBRE流式细胞仪核心获得。该CyTOF平台将用于临床 和基础研究人员在罗得岛的状态，并显示出高水平的支持和奉献精神， COBRE流式细胞术核心的成功。凭借最先进的仪器和核心技术， 扩展计划旨在扩大临床研究项目的纳入，我们的核心定位良好， 继续增长，实现长期财务可持续性。