Pathology and Biobanking

摘要 病理学和生物库核心(PBC)支持贝勒医学院的努力 26年，为孢子研究者和其他人提供全面的病理服务。全 这一孢子应用中的项目建议使用人类生物样品进行分析开发和 回溯性和/或前瞻性临床试验，此共享资源核心是为满足需求而构建的 用于在这种情况下高质量地集中存放和分发组织标本。我们有专业知识 和专职人员进行所有常规组织学研究，免疫组织化学，免疫荧光，组织 微阵列制备和宏观/微观解剖技术。此外，酷睿还支持高级 显微镜和数字成像能力。所有项目也将受益于我们最近扩大的国家 ART分子诊断平台和高复杂性分析开发，包括定量PCR， 基于微流控的数字液滴聚合酶链式反应(DdPCR)、下一代测序(NGS)。 该核心将为项目提供以下服务，并与本提案中的其他核心集成： 1)集中资源获取、存储和利用人类生物样品用于癌症研究；2)协调 并管理孢子的病理活动，并确保项目调查人员随时可以访问 专门的病理学专业知识和解释；以及3)设计和验证基于生物标记物的测试(基因组 和/或蛋白质组学)，支持所有项目阶段，包括临床验证和报告的孢子- 以临床试验为基础。 孢子受益于组件之间的集成交互作用，该核心将密切合作 与其他核心，特别是用于项目设计和相关分析的信息学和统计核心(ISC)。 此外，我们将与ISC合作，协助选择和实施生物菌素储存库， 跟踪和管理软件，跨越所有孢子组件。我们会与政府当局合作 和倡导核心管理实验室资源，并协助职业发展实习生 进行化验开发和实验分析方面的教育。核心将协助拟议的和资助的试点项目 具有探索性分析设计和技术验证支持的研究。病理学与生物库的核心 实验室正在寻求CLIA-和CAP-认证，以满足该孢子在 贝勒医学院。

Abstract The Pathology and Biobanking Core (PBC) has supported the efforts of the Baylor College of Medicine Breast SPORE for 26 years, offering comprehensive pathology services to SPORE investigators and others. All projects in this SPORE application propose the use of human biospecimens in assay development and for retrospective and/or prospective clinical trials, and this shared resource core is structured to meet the demands for high-quality, centralized banking and distribution of tissue specimens within this context. We have expertise and dedicated personnel for all routine histological studies, immunohistochemistry, immunofluorescence, tissue microarray preparation, and macro/micro-dissection techniques. Additionally, the Core supports advanced microscopy and digital imaging capability. All projects will also benefit from our recent expansion of state-of-the art molecular diagnostic platforms and high-complexity assay development, including quantitative PCR, microfluidics-based digital droplet PCR (ddPCR), next generation sequencing (NGS). The Core will provide the following services to projects and integration with other cores in this proposal: 1) a centralized resource to acquire, store, and utilize human biospecimens for cancer research; 2) To coordinate and manage the pathology activities of the SPORE, and ensure that Project Investigators have ready access to specialized pathology expertise and interpretation; and 3) to design and validate biomarker-based tests (genomic and/or proteomic) that support all project stages, including clinically-validated and reported assays for SPORE- based clinical trials. The SPORE benefits from integrated interactions between components, and this Core will work closely with other cores, particularly the Informatics and Statistics Core (ISC) for project design and correlative analyses. Further we will collaborate with the ISC to assist with selection and implementation of biospecimen repository, tracking and management software that spans all SPORE components. We will work with the Administration and Advocacy Core in management of laboratory resources and assist Career Development trainees with education in assay development and experimental analysis. The Core will assist proposed and funded Pilot Studies with exploratory assay design and technical validation support. The Pathology and Biobanking Core Laboratory is seeking both CLIA- and CAP-certification to meet Precision Medicine initiatives of this SPORE at Baylor College of Medicine.