Flow Cytometry Shared Resource

流式细胞仪共享资源 项目摘要/摘要 流式细胞仪共享资源成立于2004年，提供现成且经济实惠的访问状态 先进的多参数细胞分析和分类能力。这个设施由大量的 用于量化单个细胞的荧光的各种流式细胞仪，通常如下 用特定的抗体进行染色。流式细胞术已经成为许多人研究癌症的重要工具 不同的目的。一些重要的应用包括肿瘤微环境的免疫图谱和 癌细胞的表型。用流式细胞术进行细胞分选是纯化细胞的关键 基因组学、蛋白质组学、代谢组学或肿瘤干细胞的分离。这一机构资源为 整个耶鲁医学院(YSM)校园，包括耶鲁癌症中心(YCC)，因此有一个关键的 大量用户支持这些大型而昂贵的仪器。 在最近的筹资期间，197个实验室使用了流式细胞术。这些用户中有94个 (48%)为青年会会员。这种共享资源最广泛地被基因组学、遗传学和 表观遗传学(GGE)和癌症免疫学(CI)研究计划。各种各样的化验方法有 该实验室还提供关于实验方案的咨询，其经验丰富的全职 导演。 流式细胞术在癌症研究中有多种应用。例如，随着癌症研究的步伐 免疫学和生物治疗的增加，定义了混合的功能和表型的异质性 细胞种群将继续是癌症研究中的关键技术。流式细胞术不仅是必不可少的 用于癌症免疫学研究，但也支持许多其他类型的研究，如肿瘤发生， 细胞信号事件、临床前模型、疗效评估、使用CRISPR的基因编辑 当然，还有基因组和RNA测序的细胞分类。显然，流式细胞术对许多 YCC内的计划。 流式细胞仪的具体目标是：(1)提供高质量、便捷的流式细胞仪服务 (2)教育青年会社群有关流式细胞术在 医学研究；以及(3)与YCC调查人员合作开发新的应用。

FLOW CYTOMETRY SHARED RESOURCE PROJECT SUMMARY/ABSTRACT Flow Cytometry Shared Resource was established in 2004 to provide ready and affordable access to state-of- the-art multi-parameter cell analysis and sorting capabilities. This facility is comprised of a large number and variety of flow cytometry instruments that quantify the fluorescence of individual cells, typically following staining with specific antibodies. Flow cytometry has become an important tool in cancer research for many different purposes. Some important applications include the immune profiling of tumor microenvironment and the phenotyping of cancer cells. Cell sorting by Flow Cytometry is critical for the purification of cells for genomics, proteomics, metabolomics, or the isolation of tumor stem cells. This institutional resource serves the entire Yale School of Medicine (YSM) campus, including Yale Cancer Center (YCC), and thus has a critical mass of users supporting these large and expensive instruments. During the most recent funding period, 197 laboratories have used Flow Cytometry. Ninety-four of these users (48%) were YCC members. This Shared Resource was most widely used by the Genomics, Genetics and Epigenetics (GGE) and the Cancer Immunology (CI) Research Programs. A wide variety of assays are performed, and the laboratory also offers consulting on experimental protocols with its experienced full-time director. Flow cytometry has multiple applications in cancer research. For example, as the pace of research into cancer immunology and biologic therapies increases, defining the functional and phenotypic heterogeneity of mixed cell populations will continue to be a critical technology in cancer research. Flow cytometry is not only essential for cancer immunology research, but also empowers numerous other types of studies such as oncogenesis, cell signaling events, preclinical models, the assessment of therapeutic efficacy, gene editing using CRISPR and of course, cell sorting for genomic and RNA sequencing. Clearly, Flow Cytometry is vital to a number of programs within YCC. The Specific Aims of Flow Cytometry are to: (1) Provide flow cytometry services that are of high quality, readily available, and at reasonable cost; (2) Educate the YCC community on the applications of flow cytometry in medical research; and (3) Collaborate with YCC investigators in the development of new applications.