Research Supplements to Promote Diversity

项目摘要 这项建议是申请补充补助金，以支持一名有才华的少数族裔博士生， Frank DeLuna将开发一种新的无标记成像系统，用于京博士的药物毒性分析 Yong Ye在圣安东尼奥德克萨斯大学的实验室。药物毒性的准确分析 由于毒副作用导致新发现的候选药物失败，因此需求迫切 导致了约30%的临床损耗，导致了巨大的经济损失 制药业。这些失败的主要原因之一可以归因于这样一个事实 大多数动物模型不能准确反映人类生理。因此，有一个 在进行药物毒性分析之前，对构建人体器官模型非常感兴趣 进行临床试验。为此，至关重要的不仅是构建能够 很好地模拟了人体组织的结构和功能，还能持续监测 人体芯片上器官模型对原位药物作用的动态行为 延长期限。父R01项目(1R01GM126571)的总体目标是解决 使用受监控的人体芯片上器官模型进行药物毒性分析的挑战性问题 具有自动化的、无标签的光学生物传感器系统，允许实时、长期、 人体心脏组织模型对不同药物反应的敏感性和动力学分析 它们的微环境。在本补充资料下为学生建议的研究活动 格兰特将利用光子晶体生物传感器开发一种新的无标签成像系统 独特的检测模式，可准确量化因药物毒性而产生的生物标志物。这个 提议的工作与父项目中的目标2相关，它还将为 在传感器的消逝场内直接成像样品以实现同时监测 由于生物标志物的结合事件，每个样品井中的反射率变化 芯片上的心脏模型，传感器表面带有生物识别探头。这种新设计将提供 药物毒性检测中生物标志物多重检测的良好替代方法。这 补充助学金将为少数民族学生提供一个深入学习的绝佳培训机会。 在生物光子学方面的知识和必要的研究技能，以及提高他的其他 能力，如写作和网络技能，为他成功的学术生涯做好了准备。

Project Summary This proposal is to apply for a supplement grant to support a talented, minority Ph.D. student, Frank DeLuna, to develop a new label-free imaging system for drug toxicity assays in Dr. Jing Yong Ye’s laboratory at the University of Texas at San Antonio. Accurate analysis of drug toxicities is in urgent demand as failures of newly discovered drug candidates due to toxic side effects have resulted in about 30% of clinical attrition, leading to enormous economic losses for the pharmaceutical industry. One of the main reasons for these failures can be attributed to the fact that most animal models fail to accurately reflect human physiology. Therefore, there is a significant interest in constructing human organ models for drug toxicity assays before proceeding with clinical trials. For that, it is critically important not only to construct small tissue chips that can well mimic the architecture and functionality of human tissues, but also to continuously monitor the dynamic behaviors of human organ-on-a-chip models in response to drugs in situ over an extended period. The overarching goal of the parent R01 project (1R01GM126571) is to address the challenging issues of drug toxicity assays by using a human organ-on-a-chip model monitored with an automated, label-free, optical biosensor system that allows for real-time, long-term, sensitive, and kinetic analyses of human cardiac tissue models in response to various drugs in their microenvironments. The proposed research activities for the student under this supplement grant is to develop a new label-free imaging system by utilizing a photonic crystal biosensor in a unique detection mode for accurate quantification of biomarkers due to drug toxicities. The proposed work is related to Aim 2 in the parent project, and it will also create a new capability for directly imaging samples in the evanescent field of the sensor for simultaneously monitoring reflective index changes in each sample well due to binding events of biomarkers secreted by a heart-on-a-chip model with biorecognition probes on the sensor surface. This new design will offer a good alternative approach for multiplex detection of biomarkers in drug toxicity assays. This supplement grant will offer an excellent training opportunity for the minority student to gain deep knowledge and necessary research skills in biophotonics as well as to improve his other capabilities, such as writing and networking skills, preparing him for a successful academic career.