Voltage imaging of astrocyte-neuron interactions

项目总结 本应用是对R01 NS113499《星形胶质细胞电压成像》的多样性补充- 塔夫茨大学克里斯·杜拉博士的“神经元相互作用”。候选人是杰奎琳·加西亚，一名预科医生 博士研究生在杜拉博士的实验室专注于星形胶质细胞电压成像和星形胶质细胞膜如何 电压影响谷氨酸的摄取。Jacqueline将使用目前正在使用的工具Dulla Lab，并使用它们 她对创伤性脑损伤如何影响星形胶质细胞膜电位及其下游的兴趣 谷氨酸信号。与R01项目官员米里亚姆·利德斯博士的讨论认为，这种方法是 并将为杰奎琳自己的独立事业提供一个发射台。杰奎琳有资格成为 作为一名拉丁裔的多元化候选人，由于她的经济背景较差。因此，她是一个 代表人数不足的多个团体的成员。这份增刊将为杰奎琳的三年研究生提供资金。 论文工作。第三年的资金将取决于第二年期间提交的NRSA申请。在这段时间里 杰奎琳将接受细胞和分子神经科学、创伤性脑损伤、癫痫、神经胶质细胞等方面的培训 生物学，还有更多。她将获得生物化学、创伤性脑损伤模型、星形胶质细胞遗传学方面的专业知识 编码电压指示器(GEVI)成像、iGluSnFR谷氨酸成像和电生理。这次培训 将引导她申请NRSA，并成为神经科学研究的领先者。加西亚女士是 致力于学术研究，重点是创伤性脑损伤。杜拉博士有丰富的经验 被授予2018年度兰迪斯杰出导师奖，并参与了 HHMI乔里亚姆奖学金通过实验室里的其他学生。这里展示的项目将给加西亚女士带来极好的 以及谷氨酸神经传递、神经元/神经胶质细胞相互作用和尖端成像方面的严格培训 医疗模式。杜拉博士和加西亚女士已经建立了结构化、严谨和定制的导师制度，并 训练计划。通过这一补充提供的资金将给杰奎琳一个机会来丰富她 研究生培训，以便她能够获得额外的培训，并创建广泛的专业网络 同事们。这一多样性补充将给杰奎琳一条通向令人兴奋的未来的明确道路 在癫痫、创伤性脑损伤和谷氨酸神经传递领域的未来研究人员。活动 展示和丰富的机会将对加西亚女士的发展和作为一名成功的 独立调查员。

PROJECT SUMMARY This application is for a diversity supplement to the R01 NS113499 “VOLTAGE IMAGING OF ASTROCYTE- NEURON INTERACTIONS” of Dr. Chris Dulla at Tufts University. The candidate is Jacqueline Garcia, a pre- doctoral student in the lab of Dr. Dulla focusing on astrocyte voltage imaging and how astrocyte membrane voltage affects glutamate uptake. Jacqueline will take the tools currently in use the Dulla Lab and use them to pursue her interests in how traumatic brain injury affects astrocyte membrane potential and downstream glutamate signaling. Discussions with the R01 program officer, Dr. Miriam Leenders, deemed this approach was “in scope” and will provide Jacqueline with a launch pad for her own independent career. Jacqueline qualifies as a diversity candidate as a Latina and due to her economically disadvantaged background. Therefore she is a member of multiple underrepresented groups. This supplement will fund Jacqueline for 3 years of her graduate thesis work. Funding for Y3 will be contingent on submission of an NRSA application during Y2. During this time Jacqueline will receive training in cellular and molecular neuroscience, traumatic brain injury, epilepsy, glial biology, and more. She will gain expertise in biochemistry, models of traumatic brain injury, astrocyte genetically encoded voltage indicator (GEVI) imaging, iGluSnFR glutamate imaging, and electrophysiology. This training will guide her towards an NRSA application and a future as a leader in neuroscience research. Ms. Garcia is devoted to academic research with a focus on traumatic brain injury. Dr. Dulla has a great deal of experience mentoring students, was awarded the 2018 Landis Award for Outstanding Mentorship, and is involved with the HHMI Gilliam Fellowship through other students in the lab. The project laid out here will give Ms. Garcia excellent and rigorous training in glutamate neurotransmission, neuron/glia interactions, and cutting-edge imaging modalities. Dr. Dulla and Ms. Garcia have developed a structured, rigorous, and customized mentorship and training plan. The funding offered through this supplement will give Jaqueline the opportunity to enrich her graduate training so that she can gain additional training and create an extensive network of professional colleagues. This diversity supplement will give Jacqueline a clear path toward an exciting future as a up and coming researcher in the field of epilepsy, traumatic brain injury, and glutamate neurotransmission. The activities laid out and the enrichment opportunities will be critical to Ms. Garcia’s development and future as a successful independent investigator.