BAF - SR Component

生物分子分析设施--项目总结 生物分子分析设施(BAF)为各种服务和 “组学”(基因组学、转录组学、蛋白质组学、类脂组学、代谢组学)和分子学中的仪器 并提供全面的生物信息学支持服务，如管道和数据分析 工具。该设施分为相互作用和支持的部分-蛋白质组学、基因组科学、 脂肪组学/代谢组学、共享仪器和生物信息学。该资源为伊利诺伊大学服务 弗吉尼亚癌症中心(UVACC)成员提供实验设计、样品处理、 具有成本效益的数据收集、数据分析以及对手稿和赠款的支持。UVACC调查人员 能够在当地获得各种服务，这些服务可以在 细胞水平，包括下一代测序，包括散装DNA/RNA-Seq、单细胞/核RNA和 ATAC-SEQ，第三代长读测序，定量基因表达，基因分型，复合蛋白 混合物，生物标志物分析，翻译后修饰，分子相互作用和动力学，脂类， 代谢物和药物分析。该设施的整合组织使UVACC成员能够 在相同或相关的样本上执行许多这样的实验。BAF内的一个关键重点领域是 为满足UVACC需求的先进仪器和实验提供通道。在过去两年内 几年来，该设施在蛋白质组学和代谢组学领域增加了两个质谱仪，并增加了两个更高的 容量NGS仪器。由于大多数BAF用户都是UVACC成员，因此与这些用户的互动 研究人员是获得新仪器和开发新技术的驱动力。这个 UVACC执行委员会和研究核心管理办公室(ORCA-医学院内部 (SOM)Dean‘s Office)密切合作，促进这种环境。为此，SOM提供了直接 每年向BAF提供预算支持(总预算约30%)，与UVACC共同支付，提供 UVACC会员以极具竞争力的价格提供高效的服务。

BIOMOLECULAR ANALYSIS FACILITY – PROJECT SUMMARY The Biomolecular Analysis Facility (BAF) provides a centralized setting for a diverse suite of services and instrumentation in the `omics' (genomics, transcriptomics, proteomics, lipidomics, metabolomics) and molecular interaction and provides comprehensive bioinformatics support services such as pipelines and data analysis tools. The facility is divided into sections that interact and support each other – Proteomics, Genomic Sciences, Lipidomics/Metabolomics, Shared Instrumentation, and Bioinformatics. The resource serves University of Virginia Cancer Center (UVACC) Members by providing expertise in experimental design, sample processing, cost-effective data collection, data analysis, and support for manuscripts and grants. UVACC investigators have access locally to a wide variety of services that can determine qualitative and quantitative changes at the cellular level, including Next Generation Sequencing, including bulk DNA/RNA-Seq, Single cell/nuclei RNA and ATAC-seq, third generation long-read sequencing, quantitative gene expression, genotyping, complex protein mixtures, biomarker profiling, post-translational modifications, molecular interactions and kinetics, lipids, metabolites, and drug analysis. The consolidated organization of the facility allows UVACC Members to perform many of these experiments on the same or related samples. One key area of focus within the BAF is providing access to advanced instrumentation and experiments that meet UVACC needs. Within the past two years, the facility added two mass spectrometers in the areas of proteomics and metabolomics and two higher capacity NGS instruments. As the majority of BAF users are UVACC Members, the interaction with these investigators is the driving force for acquiring new instrumentation and developing new techniques. The UVACC Executive Committee and Office of Research Core Administration (ORCA – within School of Medicine (SOM) Dean's Office) work closely together to foster this environment. To that end, the SOM provides direct budget support to the BAF every year (~30% total budget), which together with the UVACC co-pay, provides UVACC Members with highly effective services at remarkably competitive prices.