Cell Biology Core

细胞生物学核心 摘要 细胞生物学核心的目标是向耶鲁大学研究中心的成员提供仪器， 技术人员，以及糖尿病相关研究领域中细胞功能分析的专业知识。这个 CORE专注于分子和细胞成像技术以及胰岛细胞功能的分析。成象 方法包括光学显微镜和电子显微镜，以及凝胶和多孔板的定量红外成像。 重点是免疫细胞化学方法和活细胞的动态光学显微镜成像。 包含荧光标记，使用标准的荧光、全内反射和共聚焦 (包括旋转磁盘)技术。此外，核心还提供了前沿技术，如电子 显微断层扫描和三种不同类型的超分辨率显微镜(受激发射 耗尽、单分子开关纳米显微镜和结构照明显微镜)。刚果民主共和国调查人员 将根据工作需要接受各种成像技术培训。胰岛细胞生物学成分 CORE提供胰岛分离、高通量并行胰岛灌流和激素动态研究 分泌物。此外，Core还提供对胰岛和其他细胞类型或孤立细胞的高分辨率呼吸测量 线粒体。预计核心提供的服务将允许澄清范围广泛的 对理解糖尿病病理生理学至关重要的细胞功能方面。

CELL BIOLOGY CORE Abstract The goal of the Cell Biology Core is to make available to members of the Yale DRC the instrumentation, technical personnel, and expertise for the analysis of cell function in areas of research related to diabetes. The Core focuses on molecular and cellular imaging techniques and on analysis of islet cell function. Imaging methods include light and electron microscopy, and quantitative infra-red imaging of gels and multiwell plates. Emphasis is given to immunocytochemical methods and to the dynamic light microscopy imaging of living cells containing fluorescent markers, using standard epifluorescence, total internal reflection, and confocal (including spinning disk) techniques. In addition, the core offers leading edge techniques such as electron microscopy tomography and three different types of super-resolution microscopy (stimulated emission depletion, single-molecule switching nanoscopy, and structured illumination microscopy). DRC investigators will be trained in various imaging techniques as required for their work. The islet cell biology component of the core offers isolation of islets, high-throughput parallel perfusion of islets, and dynamic studies of hormone secretion. As well, the Core offers high resolution respirometry of islets and other cell types or of isolated mitochondria. It is anticipated that the services provided by the Core will permit the elucidation of wide-ranging aspects of cell function that are critical to understanding diabetes pathophysiology.