Transgenic Mouse and Embryonic Stem Cell Facility

摘要 芝加哥大学医学综合癌症中心(UCCCC)转基因小鼠/胚胎 干细胞设施(TMESCF)提供全面的基因工程服务，以改变 实验室小白鼠。UCCCC的研究人员开发和利用了各种不同的癌症小鼠模型 了解癌症作为一种疾病的病因、进展和治疗。这些方法尤其是 重要的是增加我们对免疫系统在肿瘤发生和发展中的作用的了解 增加对影响肿瘤转移的因素的了解，以及对癌症其他方面最好的知识 以整只动物为模型。该设施提供专门的技术来产生基因工程 通过显微注射小鼠胚胎和通过在胚胎中使用基因打靶/编辑 干细胞。随着新的基因组编辑方法的出现，对这些服务的需求更大 比以往任何时候都多。特别是，我们教职员工对CRISPR/Cas9基因组编辑和TARGATT站点的需求- 定向转基因技术在过去几年里呈指数级增长，因为我们成功地引入了 这些技术在2013年推出。转基因小鼠/胚胎干细胞设施使我们的研究人员能够 在技术前沿开发癌症的小鼠模型，例如，CRISPR/Cas9，允许 美国将更快、更广泛地引入标签、特定的缺失/突变和其他基因变化 规模比过去更大。拥有这样的专用设施也使我们的调查人员在 这个快速发展的领域，获得关于可以开发的模型类型的非常有价值的建议，并学习 来自TMESCF正在测试的各种新的和创新的方法。

ABSTRACT The University of Chicago Medicine Comprehensive Cancer Center (UCCCC) Transgenic Mouse/Embryonic Stem Cell Facility (TMESCF) provides comprehensive genetic engineering services to alter the genome of the laboratory mouse. UCCCC investigators develop and exploit a variety of different mouse models of cancer to understand the etiology, progression and treatment of cancer as a disease. These approaches are particularly important to increase our understanding of the role of the immune system in tumorigenesis, and to develop increased knowledge of the factors affecting tumor metastasis, as well as other aspects of cancer that are best modeled in the whole animal. The Facility provides specialized technology to generate genetically engineered mice through the microinjection of mouse embryos and through the use of gene targeting/editing in embryonic stem (ES) cells. With the advent of new genome editing approaches, the demand for these services is greater than ever. In particular, demand from our faculty for CRISPR/Cas9 genome editing and TARGATT site- directed transgenics has grown exponentially over the past few years following our successful introduction of these technologies in 2013. The Transgenic Mouse/Embryonic Stem Cell Facility allows our investigators to develop mouse models of cancer at the cutting-edge of technology, with CRISPR/Cas9, for example, allowing us to introduce tags, specific deletions/mutations, and other genetic changes more rapidly and on a broader scale than in the past. Having such a dedicated Facility also allows our investigators to remain competitive in this rapidly evolving field, obtain much valued advice on the types of models that can be developed, and learn from the various new and innovative approaches being tested in the TMESCF.