DanFreez: Zebrafish Genetically-Optimized for Cryogenic Storage of Embryos

摘要： 与线虫和老鼠不同，市场上缺乏对斑马fish品系进行冷藏的方法。我们建议 利用环境和基因c因子在冷冻或接近冷冻的条件下冷藏可用胚胎。 在目标1中，我们将探索利用瞬时蛋白表达来产生与厌水生物状态相关的蛋白质。 在目标2中，我们探索了诱导Killifish滞育相关基因作为促进冷藏的一种机制。 生死存亡。在目标3中，我们将着眼于c基因和环境因素的组合，这些因素促进了 斑马fi来自冷藏的胚胎。实现这些目标中的任何一个，从而从寒冷中获得高的生存产量 然后，存储可以作为基因整合系在第二阶段开发，然后可以作为一种广泛的商业化 产品或服务，以帮助研究人员在他们的生物库eff奥特与他们的斑马fish线。

Summary: Unlike C. elegans and mice, methods for cold storage of zebrafish lines are lacking in the marketplace. We propose the use of environmental and gene c factors to enable cold storage of viable embryos at frozen or near freezing condi ons. In Aim 1 we will explore the use of transient protein expression to produce proteins associated with anhydrobio c status. In Aim 2 we explore the induc on of genes involved in diapause in killifish as a mechanism to promote cold-storage survival. In Aim 3, we will look at combina ons of gene c and environmental factors that promote reanima on of zebrafish embryos from cold storage. Achievement of any one of these aims that result in high viability yields from cold storage can then be developed in Phase II as a gene cally integrated line, which can then be broadly commercialized as a product or service to aid researchers in their biobanking efforts with their zebrafish lines.