UTHSC Regional Biocontainment Laboratory: Upgrade of Facility and Building System

项目摘要 田纳西大学健康科学中心(UTHSC)建议升级设施并扩大 位于田纳西州孟菲斯的现有UTHSC区域生物遏制实验室(RBL)的研究服务 支持内部和外部研究人员对新出现的病原体进行基础和翻译研究，并 选择利用小动物的药剂。目标1：硬件升级和楼宇自动化系统软件升级 这是自2009年以来一直存在的，是我们利用这一融资机会的首要任务，并将使UTHSC RBL将继续良好地运营到未来。在目标2中，我们请求提供支持以扩大现有基础设施 为了利用我们现有的设施达到其设计能力，增加了笼子和机架。重要的是，一位 我们计划的组成部分是升级我们的隔膜，以容纳普罗万提斯研究所，为临床前提供支持 服务。在目标3和4中，我们要求提供全动物成像和免疫分析设备，以 加强我们在用户可用的终端方面满足研究服务需求的能力； 内部和外部。总而言之，这项建议旨在：(1)升级和更换过时或过时的产品 建筑物管理系统的组成部分；(2)扩大老鼠以外的小动物的基础设施， (3)升级现有动物成像能力，设立一个动物生物安全3级(ABSL3)实验室 对于IVIS/CT成像系统，(4)使用专门的ABSL-3区域进行临床前服务 实践(GLP)监测；和(5)目前无法提供的实现免疫学分析的设备(ELISPOT) 并用光谱流式细胞仪取代过时的流式细胞仪设备。

Project Summary The University of Tennessee Health Sciences Center (UTHSC) proposes to upgrade facilities and broaden research services in the existing UTHSC Regional Biocontainment Laboratory (RBL) located in Memphis, TN to support internal and external investigators in basic and translational research with emerging pathogens and select agents utilizing small animal. In Aim 1, upgrading the hardware and building automation system software that has been in place since 2009 is our top priority with this funding opportunity and will allow the UTHSC RBL to operate continuously well into the future. In Aim 2, we request support to expand existing infrastructure in order to use our current facility to its design capacity with additional caging and racks. Importantly, a major component of our plan is to upgrade our vivarium to house the Provantis Instem to provide support for preclinical services. In Aim 3 and 4, we request equipment for whole animal imaging and immunological analyses to strengthen our ability to meet the research service demands in terms of endpoints available to users; both internally and externally. In summary, this proposal seeks to: (1) upgrade and replace outdated or obsolete components of the building management system; (2) expand infrastructure for small animals other than mice, (3) upgrade current animal imaging capabilities and dedicate one animal biosafety level 3 (ABSL3) laboratory for the IVIS/CT imaging system, (4) enable preclinical services with a dedicate ABSL-3 area for Good Laboratory Practice (GLP) monitoring; and (5) equipment to enable immunological analyses currently not available (elispot) and replace an obsolete flow cytometry equipment with a spectral flow cytometer.