Dietary Supplement of n-3 PUFA to Control Corneal Inflammation

标题：n-3多不饱和脂肪酸控制角膜炎症的膳食补充 摘要：角膜作为眼睛最外层的组织，经常受到角膜擦伤、穿孔等损伤。 导致角膜发炎的伤口、化学烧伤和热烧伤。角膜炎症也是 遗传性疾病，如圆锥角膜，长期配戴隐形眼镜，以及外科角膜移植。 炎症失衡和伤口愈合不当(溶解)会导致角膜瘢痕和 导致失明的新生血管(NV)。全世界高达四分之一的失明病例是 可归因于疤痕形成的角膜混浊，或纤维化，代表着巨大的经济和社会 负担。角膜纤维化发展的主要原因是由于伤口愈合过程中的缺陷 炎症及其消退的失衡。目前，还没有FDA批准的药物来促进适当的 角膜伤口愈合。我们推测鱼油中的n-3多不饱和脂肪酸(PUFA) 补充剂将通过减少慢性炎症和增加角膜新生血管来抑制角膜纤维化和新生血管 解决和愈合伤口。 在炎症和组织愈合过程中，它是N-3多不饱和脂肪酸(如二十碳五烯酸、EPA和 二十二碳六烯酸(DHA)，可产生多种生物活性脂类介体(如溶血素、神经保护素 和甲基树脂)，在消炎和组织愈合方面发挥重要作用。虽然数量很大 关于n-3多不饱和脂肪酸(DHA和EPA)在许多人类全身系统中的益处的研究数据是可用的 在各种疾病中，n-3多不饱和脂肪酸在角膜愈合和恢复视力中的作用尚未被研究。我们建议 测定日粮中添加欧米茄Rx2鱼油n-3多不饱和脂肪酸的效果(区域实验室) 预防小鼠角膜损伤模型中的角膜瘢痕和新生血管。我们预计我们的研究最终将 翻译成人类补充的n-3多不饱和脂肪酸有助于平衡炎症和 提高角膜的分辨率和创面愈合，这些角膜由于潜在的疾病容易形成疤痕和新生血管。 遗传原因，戴隐形眼镜或角膜移植手术，从而将防止形成 永久的伤疤和失明。

Title: Dietary Supplement of n-3 PUFA to Control Corneal Inflammation Abstract: Cornea being the outer most tissue in the eye suffers regular injuries from corneal abrasions, puncture wounds, chemical and thermal burns that induce corneal inflammation. Corneal inflammation is also integral to genetic diseases like keratoconus, long-term wearing of contact lenses, and surgical corneal transplantation. Imbalances in inflammation and improper wound-healing (resolution) causes corneal scar and neovascularization (NV) that leads to blindness. Up to one fourth of all cases of blindness worldwide are attributable to corneal opacities generated by scarring, or fibrosis, representing a huge economic and societal burden. The primary cause of corneal fibrosis development is defective wound healing process due to imbalances in the inflammation and its resolution. Currently, there is no FDA-approved drug that facilitates proper corneal wound healing. We hypothesize that n-3 polyunsaturated fatty acids (PUFAs) from Fish Oil dietary supplementation will inhibit corneal fibrosis and NV by reducing chronic inflammation and augmenting resolution and wound-healing. During inflammation and tissue healing, it is the N-3 PUFAs (such as eicosapentaenoic acid, EPA, and docosahexaenoic acid, DHA) that generates several bioactive lipid mediators (such as resolvins, neuroprotectins and maresins) that play essential roles in resolution of inflammation and tissue healing. Although substantial research data is available regarding the benefits of n-3 PUFAs (DHA and EPA) in many human systemic diseases, the role of n-3 PUFAs in corneal healing and restoration of vision has not been studied. We propose to determine the efficacy of dietary supplementation of n-3 PUFA from OmegaRx2 Fish Oil (Zone Labs) to prevent corneal scar and NV in mouse model of corneal injuries. We anticipate our study will ultimately translate in to human augmentative dietary supplement of n-3 PUFAs to help balance the inflammation and improve the resolution and wound-healing in the corneas that are prone to develop scar and NV due to underlying genetic causes, contact lens wearing or corneal transplantation surgery and thus will prevent formation of permanent scar and blindness.