EMRSHN: Exploring the Modulatory Role of Sec Hormones Along the Neuromechanical Axis in Females

项目摘要 基于我们目前的R 01，我们建议描述全身激素的作用 浓度对基本骨骼肌特性的影响。最初，年轻和健康 将招募女性手术患者以确定肌肉之间的关联映射， 纤维成分和E2浓度，使用组织学和蛋白质/基因表达的骨骼肌 肌肉活检接下来，将招募年轻健康的女性受试者， 肌肉纤维组成在月经周期期间的有限时间窗口内变化，使用 最先进的7特斯拉扫描仪来进行磁共振波谱分析最后，年轻的， 将招募健康女性受试者以确定内分泌的潜在影响。 通过监测运动期间循环的肌萎缩侧索硬化和肌肉活动来了解肌肉可塑性。 如果成功完成，这些目标将揭示一个主题之间的关系 内分泌状态和基本肌肉表型的急性变化以及相关变化 神经肌肉控制这些目标合在一起有可能阐明 潜在的生物学机制，可能会导致肌肉骨骼损伤中的性别差异 并指导未来的伤害预防策略研究。

Project Summary Building off of our current R01, we propose to characterize the effect of systemic hormone concentrations on basic skeletal muscle characteristics. Initially, young and otherwise healthy female surgical patients will be recruited to determine an associative mapping between muscle fiber composition and E2 concentration using histology and protein/gene expression of skeletal muscle biopsies. Next, young and healthy female subjects would be recruited to identify potential muscle fiber composition changes over a limited time window during the menstrual cycle, using a state-of-the-art 7 Tesla scanner to perform magnetic resonance spectroscopy. Finally, young and healthy female subjects would be recruited to identify the potential effect of the endocrinological state on muscle plasticity by monitoring circulating myomiRs and muscle activity during exercise. If successfully completed, these aims would reveal a relationship between a subject’s endocrinological state and acute changes in basic muscle phenotype and the associated changes in neuromuscular control. Taken together these aims have the potential to elucidate the underlying biological mechanisms that may drive the sex-disparity seen in musculoskeletal injury and to guide future research in injury prevention strategies.