Identification and Characterization of Nobel Genetic Mechanisms in Alcohol Use Disorder and Excessive Drinking

项目摘要 本补充申请要求将资助K99 AA 027835延长一年，并提供额外资金，用于 阿曼达·巴克利-利文森医生的工资补贴由于不可预见的和 COVID-19疫情对受资助项目造成的极端影响以及随后对 研究活动。该项目的目标没有改变，但需要更多的时间来完成 计划的工作。不需要额外的研究资金，所有实验和动物费用将由我们支付。 父母的恩赐。在拟议的一年延长期内，Barkley-Levenson博士将继续随访 使用突变小鼠系评价新的GWAS命中问题酒精使用。她将完成 通过测量乙醇对颅内神经元的影响对Fut 2基因敲除小鼠进行综合行为表型分析 自我刺激和戒断相关的饮酒升级和慢性 间歇性乙醇暴露模型（Aim 1）。巴克利-利文森博士还将完成行为表型分析 用于初步GWAS分析的AIL小鼠的初始队列（Aim 2）。这些实验是不可能的， 过去一年，由于州和大学对非必要研究的限制以及对亲自工作的限制， 现在可以根据当前的指导方针继续进行。预计不会再有拖延。在延长 在此期间，巴克利-利文森博士将继续从事职业发展活动，包括写作和出版 论文，在研究会议上发表，指导本科生研究助理，申请教师 工作，并努力及时过渡到一个独立的位置。

Project summary This supplement application requests a one-year extension of grant K99AA027835 with additional funds for salary support for Dr. Amanda Barkley-Levenson. This supplement is needed due to the unforeseen and extreme impacts on the funded project created by the COVID-19 pandemic and subsequent restrictions on research activities. The aims of this project have not changed, but additional time is needed to complete the planned work. No additional research funds are requested, and all experimental and animal costs will be paid by the parent grant. During the proposed one-year extension, Dr. Barkley-Levenson will continue follow-up evaluation of novel GWAS hits for problematic alcohol use using mutant mouse lines. She will complete comprehensive behavioral phenotyping of Fut2 knockout mice by measuring ethanol effects on intracranial self-stimulation, and withdrawal-associated escalation of drinking and negative affective changes in the chronic intermittent ethanol exposure model (Aim 1). Dr. Barkley-Levenson will also complete behavioral phenotyping of an initial cohort of AIL mice for preliminary GWAS analysis (Aim 2). These experiments were not possible in the past year due to state and university restrictions on non-essential research and limits on in-person work but are now able to proceed under current guidelines. No further delays are anticipated. During the extension period, Dr. Barkley-Levenson will continue with career development activities including writing and publishing papers, presenting at research conferences, mentoring undergraduate research assistants, applying for faculty jobs, and working towards a timely transition to an independent position.