Proteomics

摘要-蛋白质组学共享资源 蛋白质组学共享资源的使命是提供经济高效、最先进的分析蛋白质组学 为癌症中心的研究人员提供服务。在过去的供资期间，核心系统因此进行了升级 这是SBP的一项重大举措，它为购买新设备提供了超过250万美元的支持。这 升级后的设备包括两个Thermo Fusion Lumos系统、Q-Exactive Plus和TSQ Quantiva系统， 所有这些都带有适当的耦合LC系统。此外，还增加了Bravo assayMap自动化功能 样品制备系统和招聘训练有素的蛋白质组科学家-现在是核心工作人员 包括三名博士级别的科学家。蛋白质组学核心提供全方位的服务，包括研究 设计、样品制备以及数据的生成、分析和解释。除了直截了当 蛋白质鉴定，核心支持涉及尖端蛋白质组学方法的工作，包括标记 和无标记定量蛋白质组学，以及聚焦和全球翻译后修饰(PTM) 分析。在过去的几年中，核心还承担了更大规模的高吞吐量项目。 核心还支持亲和纯化耦合到质谱仪(APMS)使用邻近标记和 研究蛋白质-蛋白质相互作用的表位标记技术。最近，核心组织密切合作 与癌症中心实验室合作实施新的蛋白质组学技术，包括组蛋白PTM图谱， 生物体液中生物标记物的发现和药物靶标的识别。在过去的资助期间，蛋白质组学 CORE已经为代表所有三个项目的41个中心成员提供了服务，这项工作支持了 至少43本与癌症相关的出版物。

ABSTRACT – PROTEOMICS SHARED RESOURCE The mission of the Proteomics Shared Resource is to provide cost-effective, state-of the-art analytical proteomics services to investigators of the Cancer Center. During the past funding period, the Core was upgraded as a result of a major initiative by SBP, which provided more than $2.5M in support for the purchase of new equipment. This upgraded equipment included two Thermo Fusion Lumos systems, Q-Exactive Plus and TSQ Quantiva systems, all with appropriate coupled LC systems. Additionally, there was addition of a Bravo AssayMap automation system for sample preparation, and the recruitment of highly trained proteomics scientists—the Core staff now includes three Ph.D.-level scientists. The Proteomics Core provides a full array of services, including study design, sample preparation, and the generation, analysis, and interpretation of data. In addition to straightforward protein identification, the Core supports work involving cutting-edge proteomics methodologies, including labeled and label-free quantitative proteomics, and both focused and global post-translational modification (PTM) analyses. In the past several years, the Core has additionally taken on larger scale high throughput projects. The Core also supports affinity purification coupled to mass spectrometry (APMS) using proximity labeling and epitope tagging technologies to study protein–protein interactions. More recently, the Core has worked closely with Cancer Center laboratories to implement new proteomics technologies, including histone PTM profiling, biomarker discovery in biofluids, and drug target identification. Over the past funding period, the Proteomics Core has provided services to 41 Center members representing all three programs, and this work has supported at least 43 cancer-relevant publications.