Microbiology and Metagenomics Core

项目摘要/摘要 微生物学与元基因组学核心 这个核心的目的是促进临床上痤疮微生物组的高通量表征。 痤疮患者样本产生自痤疮研究翻译中心(CORT)，并推动 对这些微生物的功能的分析超越了基本的基因组特征。新一代测序仪 改变了微生物组研究，但已被证明不足以理解 皮肤中的这种微生物遗传信息。痤疮微生物群已被部分描述，但 而不了解这些不同微生物的产物如何影响痤疮的病理生理学。这个 核心董事、核心副董事和该核心的员工具有丰富的世代和 分析元基因组数据，并成功开发了一组功能屏幕，以使 分析目标细胞如何对微生物的代谢产物作出反应。这需要研究方面的专业知识。 痤疮假单胞菌，痤疮中的主要细菌种类，厌氧和挑剔，因此需要专业知识 与世隔绝和文化。痤疮杆菌产生一系列发酵代谢物和其他产品，可以 改变宿主免疫反应和其他生物体的生长。这个核心将产生细菌库 从皮肤中提取生物，从皮肤拭子中进行元基因组分析，并从功能上筛选文库 它们能够改变与皮肤相关的成纤维细胞、角质形成细胞和免疫细胞的免疫反应 并分析这些观察结果以产生假说以进行进一步的测试。核心也将 与加州大学洛杉矶分校的转录组核心和脂肪组筛查密切合作，进一步整合数据进行验证 和发现力。这一能力将把数据的效用转化为CORT的成员。

PROJECT SUMMARY/ABSTRACT Microbiology and metagenomics core The purpose of this core is to facilitate high-throughput characterization of the acne microbiome from clinical samples of acne patients generated from the Acne Center of Research Translation (CORT), and to advance analysis of the function of these microbes beyond basic genomic characterization. Next-generation sequencers have transformed microbiome research but have proven insufficient to understand the biologic relevance of this microbial genetic information in the skin. The acne microbiome has been partially characterized but without understanding of how products of these diverse organisms influence the pathophysiology of acne. The Core Director, Core Associate Director, and staff of this core have extensive experience in the generation and analysis of metagenomic data and have successfully developed a panel of functional screens to enable analysis of how target cells respond to the metabolic products of microbe. This requires expertise in the study of C. acnes, the predominant bacterial species in acne that is anaerobic and fastidious, thus requiring expertise for isolation and culture. C. acnes produces a range of fermentation metabolites and other products that can alter host immune responses and the growth of other organisms. This core will produce libraries of bacterial organisms from the skin, perform metagenomic analysis from skin swabs, and functionally screen libraries for their capacity to alter the immune response of fibroblasts, keratinocytes and immunocytes relevant to the skin of acne subjects, and analyze these observations to generate hypothesis for further testing. The core will also work closely with the transcriptomics core and lipidomic screens at UCLA to further integrate data for validation and discovery. This ability will transform the utility of the data to members of the CORT.