Islet and Pancreas Analysis Core

胰岛和胰腺分析核心：项目摘要/摘要 胰岛和胰腺分析中心(IPA)成立于2007年，是由于对小鼠不断增长的需求而建立的 来自范德比尔特糖尿病研究中心(VDRC)研究人员的胰岛。在上一个供资周期中，IPA CORE向47名不同的调查员(40名范德比尔特和7名外部非营利组织)提供服务，协助 4120个程序。VDRC内的研究项目涵盖了胰岛生物学领域， 在正常的动态平衡和疾病中的发育和功能，包括广泛的胰岛相关 从细胞内信号、生理反应和适应、1型免疫学的过程 糖尿病、丰富的胰岛转录因子的功能和正常激素分泌的维持 身份和细胞命运。在这些领域进行严谨研究的关键是能够有力地分离小鼠胰岛 并检测小鼠和人胰岛的复杂生理功能。范德比尔特一直是一个 在开发胰岛功能分析方面处于全国领先地位，NIDDK支持的选择证明了这一点 综合胰岛分配计划(IIDP)，以容纳人类胰岛表型计划(HIPP)，网址为 范德比尔特在IPA核心主任Marcela Brissova的指导下。希普在范德比尔特的位置 极大地受益于VDRC调查人员，使新开发的程序能够无缝实施 通过IPA核心。除了提供功能分析外，IPA Core还提供VDRC调查人员 采用高分辨率全幻灯片图像扫描仪，可同时分析两种组织结构 和单细胞形态。许多VDRC调查人员利用成像基础设施和相关 分析工具，在这次竞争更新中，IPA核心建议将多路成像添加到 执行高影响、基于组织的研究。虽然获取这项技术的成本太高，而且在技术上 对于单个实验室来说，将其作为核心服务提供将显著增强组织学的影响 对老鼠和人类组织的分析。IPA核心也在与动物新陈代谢密切合作 生理学核心(AMPC)以标准化胰岛移植技术，这是一种宝贵的实验资源 将补充现有的体外测试，并允许更多的研究人员进行体内研究。通过ITS IPA核心集中了资源和专业知识，为VDRC调查人员提供了可靠和标准化的 促进与胰岛生物学、细胞信号和新陈代谢相关的发现的程序。接下来的 IPA核心的卓越对于维持胰腺和胰岛领域的严谨和有影响力的工作至关重要 VDRC调查人员的发育和生理学。

Islet and Pancreas Analysis Core: Project Summary/Abstract The Islet and Pancreas Analysis (IPA) Core was established in 2007 as a result of growing demand for murine islets from Vanderbilt Diabetes Research Center (VDRC) investigators. Over the last funding cycle, the IPA Core provided services to 47 different investigators (40 Vanderbilt and 7 external nonprofit) by assisting with 4,120 procedures. Research programs within the VDRC encompass the fields of pancreatic islet biology, development, and function in normal homeostasis and disease, including a broad spectrum of islet-related processes raging from intracellular signaling, physiological response and adaptation, the immunology of type 1 diabetes, the function of islet-enriched transcription factors, and the maintenance of normal hormone-secreting status and cell fate. Essential to rigorous research in these areas is the ability to robustly isolate murine islets and to assay the complex physiological function of both murine and human islets. Vanderbilt has been a national leader in developing islet functional assays, as evidenced by the selection of the NIDDK-supported Integrated Islet Distribution Program (IIDP) to house the Human Islet Phenotyping Program (HIPP) at Vanderbilt under direction of Marcela Brissova, IPA Core Director. The HIPP's location at Vanderbilt has greatly benefitted VDRC Investigators, allowing newly developed procedures to be seamlessly implemented through the IPA Core. In addition to offering functional assays, the IPA Core also provides VDRC investigators with high-resolution, whole-slide image scanners that enable simultaneous analysis of both tissue architecture and single cell morphology. Numerous VDRC investigators utilize the imaging infrastructure and associated analytical tools, and in this competitive renewal, the IPA Core proposes the addition of multiplex imaging to perform high impact, tissue-based studies. While access to this technology is cost-prohibitive and technically challenging for individual labs, offering it as a core service will significantly enhance the impact of histological analysis of mouse and human tissue. The IPA Core is also working closely with the Animal Metabolic Physiology Core (AMPC) to standardize islet transplantation techniques, a valuable experimental resource that will complement existing ex vivo assays and allow more investigators to perform in vivo studies. Through its centralized resources and expertise, the IPA Core provides VDRC investigators with reliable and standardized procedures that facilitate discoveries related to islet biology, cell signaling, and metabolism. The continued excellence of the IPA Core is crucial to sustain rigorous and impactful work in the areas of pancreas and islet development and physiology by VDRC investigators.